The Effect of Donor Antigen-pulsed Dendritic Cells on Survival of Skin Allograft in a Rat Model.
- Author:
Seok Chan EUN
1
;
Byung Jun KIM
;
Jin Hee KIM
;
Chan Yeong HEO
;
Rong Min BAEK
;
Hak CHANG
;
Kyung Won MINN
Author Information
1. Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine, Gyeonggi, Korea. sceun@snubh.org
- Publication Type:Original Article
- Keywords:
Skin allograft;
Dendritic cell;
Immune tolerance
- MeSH:
Animals;
Antigen-Presenting Cells;
Dendritic Cells;
Graft Survival;
Humans;
Immune Tolerance;
Immunosuppression;
Isoantigens;
Rats;
Rejection (Psychology);
Skin;
Tacrolimus;
Tissue Donors;
Transplantation, Homologous;
Transplants;
Veins
- From:Journal of the Korean Society of Plastic and Reconstructive Surgeons
2008;35(4):367-372
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Prevention of acute rejection in skin allografts without continuous immunosuppression lacks reports in worldwide literature. Needs for chronic immunosuppression preclude the use of tissue allograft as a routine surgical reconstructive option. Recently dendritic cells(DC) gained considerable attention as antigen presenting cells that are also capable of immunologic tolerance induction. This study assesses the effects of alloantigen-pulsed dendritic cells in induction of survival increase in a rat skin allograft model. METHODS: Recipient-derived dendritic cells were harvested from rat whole blood and cultured with GM- CSF(200ng/mL) and IL-4(8ng/mL) for 2 weeks. Then donor-specific alloantigen pulsed dendritic cells were reinjected into tail vein before skin graft. The rat dorsal skin allografts were transplanted in 5 subgroups. Groups: I) untreated, II) anti-lymphocyte serum(ALS, 0.5 mL), III) FK-506(2mg/kg), IV) DCp, VI) DCp and FK- 506. Graft appearance challenges were assessed postoperatively. RESULTS: The group V(DC and FK-506 treated) showed longest graft survival rate(23.5 days) than other groups; untreated(5.8 days), ALS(7.2 days), FK-506 (17.5 days), DCp(12.2 days). CONCLUSION: Donor antigen pulsed host dendritic cell combined with short-term immunosuppression prolong skin allograft survival and has potential therapeutic application for induction of donor antigen specific tolerance.
