High levels of soluble herpes virus entry mediator in sera of patients with allergic and autoimmune diseases.
- Author:
Hyo Won JUNG
1
;
Su Jin LA
;
Ji Young KIM
;
Suk Kyeung HEO
;
Ju Yang KIM
;
Sa WANG
;
Kack Kyun KIM
;
Ki Man LEE
;
Hong Rae CHO
;
Hyeon Woo LEE
;
Byungsuk KWON
;
Byung Sam KIM
;
Byoung Se KWON
Author Information
1. The Immunomodulation Research Center and Department of Biological Sciences, University of Ulsan, Ulsan 680-749, Korea. bskwon@mail.ulsan.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
asthma;
atopic;
autoimmune diseases;
dermatitis;
inflammation mediators;
rheumatoid arthritis;
tumor necrosis factor
- MeSH:
Animals;
Antibodies, Monoclonal/immunology;
Antibody Specificity;
Arthritis, Rheumatoid/blood/immunology;
Asthma/blood/immunology;
Autoimmune Diseases/*blood/immunology;
Cell Division;
Cell Line;
Dermatitis, Atopic/blood/immunology;
Female;
Flow Cytometry;
Humans;
Hypersensitivity/*blood/immunology;
Lymphocyte Culture Test, Mixed;
Mice;
Mice, Inbred BALB C;
Receptors, Tumor Necrosis Factor/*blood/immunology;
Receptors, Tumor Necrosis Factor, Member 14;
Receptors, Virus/*blood/immunology;
Solubility
- From:Experimental & Molecular Medicine
2003;35(6):501-508
- CountryRepublic of Korea
- Language:English
-
Abstract:
Herpes virus entry mediator (HVEM) is a newly discovered member of the tumor necrosis factor receptor (TNFR) superfamily that has a role in herpes simplex virus entry, in T cell activation and in tumor immunity. We generated mAb against HVEM and detected soluble HVEM (SHVEM) in the sera of patients with various autoimmune diseases. HVEM was constitutively expressed on CD4(+)and CD8(+)T cells, CD19(+)B cells, CD14(+)monocytes, neutrophils and dendritic cells. In three-way MLR, mAb 122 and 139 were agonists and mAb 108 had blocking activity. An ELISA was developed to detect sHVEM in patient sera. sHVEM levels were elevated in sera of patients with allergic asthma, atopic dermatitis and rheumatoid arthritis. The mAbs discussed here may be useful for studies of the role of HVEM in immune responses. Detection of soluble HVEM might have diagnostic and prognostic value in certain immunological disorders.
