Identification of IgE binding components of two major weed pollens, ragweed and mugwort.
10.4168/aard.2014.2.5.337
- Author:
Moon Gyung YOON
1
;
Mi Ae KIM
;
Hyun Jung JIN
;
Yoo Seob SHIN
;
Hae Sim PARK
Author Information
1. Department of Allergy & Clinical Immunology, Ajou University School of Medicine, Suwon, Korea. hspark@ajou.ac.kr
- Publication Type:Original Article
- Keywords:
Ambrosia;
Artemisia;
Allergens;
Cross reactions
- MeSH:
Allergens;
Ambrosia*;
Artemisia*;
Cross Reactions;
Electrophoresis;
Enzyme-Linked Immunosorbent Assay;
Humans;
Immunoglobulin E*;
Korea;
Pollen*;
Rhinitis;
Rhinitis, Allergic, Seasonal;
Seasons;
Sodium
- From:Allergy, Asthma & Respiratory Disease
2014;2(5):337-343
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Ragweed and mugwort pollens are the major weed allergens that cause pollinosis in Korea. The IgE-binding components to these 2 pollens and their cross-reactivity have not been reported in Korea, while several reports had been made in Western countries. We investigated IgE-binding components to ragweed and mugwort pollens and their allergenic relationship in patients sensitive to the 2 pollens. METHODS: We enrolled 33 allergic rhinitis patients with typical seasonal pollinosis symptoms in autumn and elevated serum specific IgE levels to ragweed and/or mugwort pollens (>10 kU/L by ImmunoCAP). The protein bands of the 2 pollen extracts were determined using sodium dodecyl sulphate-polyacrylamide gel electrophoresis, and IgE immunoblot analysis was performed to determine the IgE-binding components of each pollen extract. Enzyme-linked immunosorbent assay (ELISA) inhibition and immunoblot inhibition tests were performed to evaluate the cross-reactivity between ragweed and mugwort pollen extracts. RESULTS: Eight IgE-binding components (9, 10, 11, 12, 27, 30, 38, and 80 kDa) were found in ragweed pollen extracts, of which 4 (38, 11, 27, and 80 kDa) were major IgE-binding components. Eight IgE-binding components (10, 14, 16, 20-24, 26-30, 42, 60-66, and 80-90 kDa) were found in mugwort pollen extracts, of which 2 components (26-30 and 20-24 kDa) were major IgE-binding components. No significant inhibitions were noted between ragweed and mugwort pollen extracts by the ELISA inhibition test. No significant changes were noted in IgE immunoblot inhibition analysis. CONCLUSION: We identified 4 major IgE-binding components (38, 11, 35, 27, and 80 kDa) in ragweed pollens and 2 major IgE-binding components (26-30 and 20-24 kDa) in mugwort pollens. No cross-reactivity was found between ragweed and mugwort pollens.
