Activity of Telomerase in Coal Worker's Autopsied Lung.
- Author:
Jin Suk CHUNG
1
;
Hae Yun NAM
;
Byoung Yong AHN
;
Kyoung Ah KIM
;
Young LIM
Author Information
1. Department of Occupational and Environmental Medicine,St. Mary's Hospital,The Catholic University of Korea, Seoul, Korea. nglim@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Coal worker;
Telomerase;
Lung cancer;
TRAP assay
- MeSH:
Cell Proliferation;
Coal*;
Fibroblasts;
Fibrosis;
Humans;
Life Cycle Stages;
Lung Neoplasms;
Lung*;
Pneumoconiosis;
Telomerase*
- From:Korean Journal of Occupational and Environmental Medicine
2002;14(4):347-352
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECT: We measured the activity of telomerase in coal workers lung tissue and found a significant increase in telomerase activity compared to the control group. Pneumoconiosis has the characteristics of fibroblast proliferation and the accumulation of collagen,thus finally causing the pathologic changes,which lead to the irreversible and progressive fibrosis of the lungs. We hypothesized that this cellular proliferation causing irreversible fibrosis may induce some elongation of the life cycle in the chromosomes and lead to further cellular immortalization. METHOD: 8 postmortem(within 24 hours)pneumo-coniotic cases were examined and their telomerase activity was compared with that of the autopsied lungs of lung cancer patients and of accident victims without any respiratory diseases. Using the extracted ribo-nucleoprotein from pneumoconiotic nodules, telomeric repeat amplification assay (TRAP)was done. RESULT: The pneumoconiotic lungs showed strong telomerase activity, similar to that of the lung cancer patients, while the control group showed no such activity. CONCLUSION: Based on the results of this study, we found that coal dust-induced cellular proliferation affects telomerase-activity clinically.
