Adherence of Candida to complete denture surfaces in vitro: A comparison of conventional and CAD/CAM complete dentures.
- Author:
Afnan F AL-FOUZAN
1
;
Lamya A AL-MEJRAD
;
Ahmed M ALBARRAG
Author Information
- Publication Type:Original Article
- Keywords: Complete denture; CAD/CAM; Candida; Adhesion; Edentulous; Roughness
- MeSH: Candida albicans; Candida*; Colon; Denture Bases; Denture, Complete*; Dentures; In Vitro Techniques*; Incidence; Microscopy, Fluorescence; Stem Cells; Stomatitis, Denture; Yeasts
- From:The Journal of Advanced Prosthodontics 2017;9(5):402-408
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: The goal of this study was to compare the adhesion of Candida albicans to the surfaces of CAD/CAM and conventionally fabricated complete denture bases. MATERIALS AND METHODS: Twenty discs of acrylic resin poly (methyl methacrylate) were fabricated with CAD/CAM and conventional procedures (heat-polymerized acrylic resin). The specimens were divided into two groups: 10 discs were fabricated using the CAD/CAM procedure (Wieland Digital Denture Ivoclar Vivadent), and 10 discs were fabricated using a conventional flasking and pressure-pack technique. Candida colonization was performed on all the specimens using four Candida albicans isolates. The difference in Candida albicans adhesion on the discs was evaluated. The number of adherent yeast cells was calculated by the colony-forming units (CFU) and by Fluorescence microscopy. RESULTS: There was a significant difference in the adhesion of Candida albicans to the complete denture bases created with CAD/CAM and the adhesion to those created with the conventional procedure. The CAD/CAM denture bases exhibited less adhesion of Candida albicans than did the denture bases created with the conventional procedure (P<.05). CONCLUSION: The CAD/CAM procedure for fabricating complete dentures showed promising potential for reducing the adherence of Candida to the denture base surface. Clinical Implications. Complete dentures made with the CAD/CAM procedure might decrease the incidence of denture stomatitis compared with conventional dentures.