Paragonimus paishuihoensis Metacercariae in Freshwater Crabs, Potamon lipkei, in Vientiane Province, Lao PDR.
10.3347/kjp.2013.51.6.683
- Author:
Shigehisa HABE
1
;
Pham Ngoc DOANH
;
Shinichiro YAHIRO
;
Nanthasane VANNAVONG
;
Hubert BARENNES
;
Peter ODERMATT
;
Gilles DREYFUSS
;
Yoichiro HORII
;
Yukifumi NAWA
Author Information
1. Department of Microbiology and Immunology, School of Medicine, Fukuoka University, Fukuoka, Japan.
- Publication Type:Original Article
- Keywords:
Paragonimus paishuihoensis;
metacercaria;
freshwater crab;
Potamon lipkei;
Lao PDR
- MeSH:
Animals;
Brachyura/*parasitology;
Cluster Analysis;
DNA, Ribosomal Spacer/chemistry/genetics;
Electron Transport Complex IV/genetics;
Fresh Water;
Laos;
Metacercariae/*isolation & purification;
Molecular Sequence Data;
Paragonimus/*isolation & purification;
Phylogeny;
Polymerase Chain Reaction;
Prevalence;
Sequence Analysis, DNA;
Sequence Homology
- From:The Korean Journal of Parasitology
2013;51(6):683-687
- CountryRepublic of Korea
- Language:English
-
Abstract:
Among Paragonimus species, P. paishuihoensis is one of the most mysterious and poorly understood species. Metacercariae are characterized by having a unique dendritically branched excretory bladder. However, the morphology of the adult worm remains unknown. To date, metacercariae of this species have been reported only in China and Thailand. In this study, we first found P. paishuihoensis metacercariae in freshwater crabs, Potamon lipkei, in Hinheub District, Vientiane, Lao PDR, with a prevalence of 77.7% and the average intensity of 10.3 (range 1-28) metacercariae per crab. The molecular data based on ITS2 and CO1 markers indicated that P. paishuihoensis from Laos and Thailand were almost completely identical and were close to members of the Paragonimus bangkokensis/Paragonimus harinasutai complex. Attempts to infect experimental animals (cats, dogs, and rats) with P. paishuihoensis were unsuccessful, suggesting that these animals might be unsuitable definitive hosts for the species. Further studies are necessary to elucidate the taxonomic status and life cycle of P. paishuihoensis.
