Modified Formalin-Ether Concentration Technique for Diagnosis of Human Strongyloidiasis.
10.3347/kjp.2013.51.6.743
- Author:
Witthaya ANAMNART
1
;
Pewpan M INTAPAN
;
Wanchai MALEEWONG
Author Information
1. Department of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, Thailand. awitthay@wu.ac.th
- Publication Type:Brief Communication ; Evaluation Studies ; Research Support, Non-U.S. Gov't
- Keywords:
Strongyloides stercoralis;
formalin-ether concentration technique;
agar plate culture;
feces;
diagnosis
- MeSH:
Animals;
Ether;
Feces/parasitology;
Formaldehyde;
Humans;
Parasitology/*methods;
Specimen Handling/*methods;
Strongyloides stercoralis/*isolation & purification;
Strongyloidiasis/*diagnosis;
Thailand
- From:The Korean Journal of Parasitology
2013;51(6):743-745
- CountryRepublic of Korea
- Language:English
-
Abstract:
We compared the efficacy and applicability of a modified formalin-ether concentration technique (M-FECT) to the conventional FECT (C-FECT) and the agar plate culture (APC) method for the detection of Strongyloides stercoralis larvae. For this purpose, we used 600 human fecal specimens collected in an endemic area of southern Thailand. In the M-FECT, we used 2 layers of wire meshes, instead of gauze, to avoid the loss by absorption/adhesion of larvae to the gauze during filtration, and we reduced the exposure time of S. stercoralis larvae in stool samples to formalin. By such simple modifications, the efficacy of M-FECT has become comparable to APC and was much better than that of C-FECT for the diagnosis of strongyloidiasis.
