Inhibitory effects of osteoprotegerin on osteoclast formation and function under serum-free conditions.
10.4142/jvs.2013.14.4.405
- Author:
Ying Xiao FU
1
;
Jian Hong GU
;
Yi Ran ZHANG
;
Xi Shuai TONG
;
Hong Yan ZHAO
;
Yan YUAN
;
Xue Zhong LIU
;
Jian Chun BIAN
;
Zong Ping LIU
Author Information
1. College of Veterinary Medicine, Yangzhou University, and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. liuzongping@yzu.edu.cn
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
activation;
differentiation;
osteoclast;
osteoprotegerin;
serum-free
- MeSH:
Acid Phosphatase/genetics/metabolism;
Animals;
Avian Proteins/*pharmacology;
Bone Marrow Cells/drug effects/*metabolism;
Cells, Cultured;
Ducks;
Embryo, Nonmammalian/drug effects/metabolism;
Isoenzymes/genetics/metabolism;
Macrophage Colony-Stimulating Factor/metabolism;
Osteoclasts/cytology/*drug effects/*metabolism;
Osteoprotegerin/*pharmacology;
RANK Ligand/metabolism;
Real-Time Polymerase Chain Reaction;
Receptor Activator of Nuclear Factor-kappa B/genetics/metabolism
- From:Journal of Veterinary Science
2013;14(4):405-412
- CountryRepublic of Korea
- Language:English
-
Abstract:
The purpose of this study was to determine whether osteoprotegerin (OPG) could affect osteoclat differentiation and activation under serum-free conditions. Both duck embryo bone marrow cells and RAW264.7 cells were incubated with macrophage colony stimulatory factor (M-CSF) and receptor activator for nuclear factor kappaB ligand (RANKL) in serum-free medium to promote osteoclastogenesis. During cultivation, 0, 10, 20, 50, and 100 ng/mL OPG were added to various groups of cells. Osteoclast differentiation and activation were monitored via tartrate-resistant acid phosphatase (TRAP) staining, filamentous-actin rings analysis, and a bone resorption assay. Furthermore, the expression osteoclast-related genes, such as TRAP and receptor activator for nuclear factor kappaB (RANK), that was influenced by OPG in RAW264.7 cells was examined using real-time polymerase chain reaction. In summary, findings from the present study suggested that M-CSF with RANKL can promote osteoclast differentiation and activation, and enhance the expression of TRAP and RANK mRNA in osteoclasts. In contrast, OPG inhibited these activities under serum-free conditions.
