The Effects of Hydroxyapatite-Chitosan Membrane on Bone Regeneration in Rat Calvarial Defects.
10.5051/jkape.2009.39.S.213
- Author:
Jung A SHIN
1
;
Jung Yoo CHOI
;
Sung Tae KIM
;
Chang Sung KIM
;
Yong Keun LEE
;
Kyoo Sung CHO
;
Jung Kiu CHAI
;
Chong Kwan KIM
;
Seong Ho CHOI
Author Information
1. Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University, College of Dentistry, Korea. shchoi726@yuhs.ac
- Publication Type:Original Article
- Keywords:
bone regeneration;
chitosan;
hydroxyapatites
- MeSH:
Animals;
Biocompatible Materials;
Bone Regeneration;
Bone Substitutes;
Chitosan;
Dentistry;
Durapatite;
Hand;
Humans;
Hydroxyapatites;
Male;
Membranes;
Nanoparticles;
Orthopedics;
Osteogenesis;
Rats;
Rats, Sprague-Dawley;
Regeneration;
Salicylamides
- From:The Journal of the Korean Academy of Periodontology
2009;39(Suppl):213-222
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Recently, interest in chitosan has increased due to its excellent biological properties such as biocompatibility, antibacterial effect, and rapid healing capacity. On the other hand, hydroxyapatite is used as a bone substitute in the fields of orthopedics and dentistry. The hydroxyapatite-chitosan (HA-CS) complex containing hydroxyapatite nanoparticles was developed for synergy of both biomaterials. The objective of this study was to evaluate the effect of hydroxyapatite (HA)-chitosan (CS) membrane on bone regeneration in the rat calvarial defect. METHODS: Eight-millimeter critical-sized calvarial defects were created in 70 male Sprague-Dawley rats. The animals were divided into 7 groups of 10 animals and received either 1) chitosan (CS) 100% membrane, 2) hydroxyapatite (HA) 30%/ CS 70% membrane, 3) HA 30% / CS 70%, pressed membrane, 4) HA 40%/ CS 60% membrane, 5) HA 50%/ CS 50% membrane, 6) HA 50%/ CS 50%, pressed membrane, or 7) a sham - surgery control. The amount of newly formed bone from the surface of the rat calvarial defects was measured using histomorphometry, following 2- or 8- week healing intervals. RESULTS: Surgical implantation of the HA - CS membrane resulted in enhanced local bone formation at both 2 and 8 weeks compared to the control group. The HA - CS membrane would be significantly more effective than the chitosan membrane in early bone formation. CONCLUSIONS: Concerning the advantages of biomaterials, the HA-CS membrane would be an effective biomaterial for regeneration of periodontal bone. Further studies will be required to improve the mechanical properties to develop a more rigid scaffold for the HA-CS membrane.
