MMP-1 promoter polymorphism in Korean with generalized aggressive periodontitis.
10.5051/jkape.2009.39.S.269
- Author:
Hyong Suk OH
1
;
Ok Su KIM
;
Young Jun KIM
;
Hyun Ju CHUNG
Author Information
1. Department of Periodontology, School of Dentistry and Dental Science Research Institute, Chonnam National University, Korea. hjchung@jnu.ac.kr
- Publication Type:Original Article
- Keywords:
aggressive periodontitis;
genetic polymorphism;
matrix metalloproteinase 1
- MeSH:
Aggressive Periodontitis;
Alleles;
Child;
DNA;
Female;
Gene Frequency;
Genotype;
Humans;
Matrix Metalloproteinase 1;
Polymerase Chain Reaction;
Polymorphism, Genetic
- From:The Journal of the Korean Academy of Periodontology
2009;39(Suppl):269-278
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The aim of this study was to investigate matrix metalloproteinase 1 (MMP-1) gene polymorphism (1G/2G at -1607 and A/G at -519) in Korean subject and to assess the association between polymorphism and periodontal status. METHODS: Forty nine generalized aggressive periodontitis (GAP) patients and 57 periodontally healthy children were recruited and genomic DNA was extracted from buccal swab. The polymorphisms of MMP-1 promoter genes were determined by polymerase chain reaction and restriction fragment length product (PCR-RFLP) method. The distribution of genotype and allele frequency was compared between 2 groups by chi-square test. RESULTS: There was a significant difference in the distribution of genotypes and frequency of alleles between the GAP and reference groups at the position - 519 of MMP-1 gene promoter (P< 0.05). Allele G carrier rate was significantly lower in GAP group than that of the reference group (P< 0.001). At the position -1607 of MMP-1 gene promoter, genotype distribution and allele frequency showed no statistically significant difference between the groups. However, in the female group, a significant difference was observed between the groups for the genotype distribution, allele frequency and allele 1G carrier rate (P< 0.05). CONCLUSIONS: The DNA polymorphism at the MMP-1 gene promoter might be associated with GAP in Korean.
