Suppressive effects of Schizandra chinensis Baillon water extract on allergy-related cytokine generation and degranulation in IgE-antigen complex-stimulated RBL-2H3 cells.
- Author:
Mi Ja CHUNG
1
;
Jeong Mi KIM
;
Sangchul LEE
;
Taewoo KIM
;
Daejung KIM
;
Jongmi BAEK
;
Taehyuk KIM
;
Jaesung LEE
;
Kyoungkon KIM
;
Jin A YOON
;
Myeon CHOE
Author Information
- Publication Type:Original Article
- Keywords: Allergic diseases; antioxidant; cytokine; RBL-2H3 cells; Schizandra chinensis Baillon
- MeSH: Asthma; beta-N-Acetylhexosaminidases; Digestion; Drug Industry; Immunoglobulin E; Immunoglobulins; Interleukin-13; Interleukins; Korea; Medicine, Traditional; Oxidative Stress; Plants; RNA, Messenger; Schisandra; Serum Albumin; Tumor Necrosis Factor-alpha; Water
- From:Nutrition Research and Practice 2012;6(2):97-105
- CountryRepublic of Korea
- Language:English
- Abstract: Schizandra chinensis Baillon is a traditional folk medicine plant that is used to treat and prevent several inflammatory diseases and cancer in Korea, but the underlying mechanisms involved in its anti-allergic activity are not fully understood. This study was designed to investigate mechanisms of anti-allergic activity of a Schizandra chinensis Baillon water extract (SCWE) in immunoglobulin E (IgE)-antigen complex-stimulated RBL2H3 cells and to assess whether gastric and intestinal digestion affects the anti-allergic properties of SCWE. Oxidative stress is an important consequence of the allergic inflammatory response. The antioxidant activities of SCWE increased in a concentration-dependent manner. RBL-2H3 cells were sensitized with monoclonal anti-dinitrophenol (DNP) specific IgE, treated with SCWE, and challenged with the antigen DNP-human serum albumin. SCWE inhibited beta-hexosaminidase release and expression of interleukin (IL)-4, IL-13, and tumor necrosis factor-alpha (TNF-alpha) mRNA and protein in IgE-antigen complex-stimulated RBL2H3 cells. We found that digested SCWE fully maintained its antioxidant activity and anti-allergic activity against the IgE-antigen complex-induced activation of RBL-2H3 cells. SCWE may be useful for preventing allergic diseases, such as asthma. Thus, SCWE could be used as a natural functional ingredient for allergic diseases in the food and/or pharmaceutical industries.
