Effects of 6-hydroxydopamine on the Adult Neurogenesis of Dopaminergic Neurons in the Mouse Midbrain.
- Author:
Tae Woo KIM
1
;
Hyun KIM
;
Woong SUN
Author Information
- Publication Type:Original Article
- Keywords: Parkinson's disease; 6-OHDA; mice; adult neurogenesis; dopaminergic neurons
- MeSH: Adult; Animals; Brain; Bromodeoxyuridine; Dopamine; Dopaminergic Neurons; Humans; Mesencephalon; Mice; Neurogenesis; Neurons; Organothiophosphorus Compounds; Oxidopamine; Parkinson Disease; Rats; Stem Cells; Substantia Nigra
- From:Experimental Neurobiology 2009;18(1):26-31
- CountryRepublic of Korea
- Language:English
- Abstract: Recently, restricted progenitor cells have been identified in the substantia nigra (SN) of the rat and mouse, raising a hope that resident stem/progenitor cells may be useful for the therapy of Parkinson's disease. However, it is controversial whether dopamine (DA) neurons can be spontaneously or injury-dependently generated from the endogenous stem cells in the adult brain. Here, we explored the neurogenesis in C57Bl/6 adult mice under the normal and neurotoxin-injured conditions. To monitor adult neurogenesis, we injected 5-bromodeoxyuridine (BrdU) 2 weeks after striatal injection of neurotoxin 6-hydroxydopamine (6-OHDA), and sacrificed the animals 6 weeks after 6-OHDA injection. Whereas the number of BrdU-labeled cells was slightly increased in ipsilateral side than contralateral side of the midbrain, none of BrdU- labeled cells, however, exhibited neuronal markers, NeuN or DCX. Instead, BrdU- labeled cells expressed glial markers such as GFAP (astrocyte), Olig2 (oligodendrocyte) and Iba-1 (microglia). Especially, larger portion of BrdU-labeled cells in the ipsilateral side exhibited microglial marker, indicating that increased cell production in response to the 6-OHDA injection is not related to the adult neurogenesis.
