Molecular Strain Typing of Legionella Isolates from Water in Cooling Towers of Big Buildings in Busan, Korea.
- Author:
Jeong Man KIM
1
;
Seok Hoon JEONG
;
Daeyoung SEO
;
Eun Hee PARK
;
Eun Ju SONG
;
Jae Cheol CHOI
;
Eun Yup LEE
;
Chulhun L CHANG
Author Information
1. Department of Laboratory Medicine, Dong-A University College of Medicine, Korea.
- Publication Type:Original Article
- Keywords:
Legionella;
Pulsed field gel electrophoresis
- MeSH:
Busan*;
Electrophoresis, Gel, Pulsed-Field;
Korea*;
Legionella pneumophila;
Legionella*;
Water*
- From:Korean Journal of Clinical Microbiology
2004;7(1):38-42
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: It is important to define a source of infection when outbreak of Legionella infections has occurred. The performance of a molecular strain typing method was evaluated for environmental and clinical isolates of Legionella pneumophila. METHODS: Thirteen environmental strains, eleven clinical isolates and one type strain (ATCC 33152) of Legionella pneumophila were used for the analysis of pulsed field gel electrophoresis. RESULTS: All 25 strains were discriminated into 21 types. Two strains isolated from different locations in a same building showed different types. Each two, four, and two strains were shown as the same PFGE patterns. CONCLUSIONS: Even though PFGE typing of Legionella pneumophila is excellent for strain differentiation, the same pattern does not necessarily indicate the same source of isolates.
