Characterization of FSC(high) Memory B Cells from Patients with Active Systemic Lupus Erythematosus.
10.4078/jkra.2007.14.3.219
- Author:
Joo Yeon JHUN
1
;
Young Joo KIM
;
Ji Hyun JU
;
Sung Hwan PARK
;
Soog Hee CHANG
;
Ho Youn KIM
Author Information
1. The Rheumatism Research Center (RhRC), Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Korea. rheuma@catholic.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Systemic lupus erythematosus;
Anti nuclear autoantibodies;
Memory B cell CD86 (B7-2);
CPG
- MeSH:
Antibodies;
Autoantibodies;
B-Lymphocyte Subsets;
B-Lymphocytes*;
Chromatin;
Enzyme-Linked Immunosorbent Assay;
Fluorescence;
Humans;
Immunoglobulin M;
Interleukin-15;
Lupus Erythematosus, Systemic*;
Memory*
- From:The Journal of the Korean Rheumatism Association
2007;14(3):219-226
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To determine phenotypic and functional characteristics of memory B cells in patients with systemic lupus erythematosus (SLE). METHODS: The percentage of memory B cell subsets in peripheral blood mononuclear cells (PBMC) from normal control (n=11), inactive (n=15) and active (n=10) SLE patients was determined by Fluorescence Activated Cell Sorter (FACS). In addition, the activation status of memory B cells was measured by the surface expression of CD86 (B7-2). The production of antibodies to chromatin and dsDNA (IgG and IgM type) by isolated memory B cell subsets was examined by enzyme-linked immunosorbent assay (ELISA). RESULTS: In this study, we analyzed 2 subtypes of memory B cells: FSC (Forward Side Scatter)(low) and FSC(high) memory B cell. The percentage of both subtypes from active and inactive SLE patients was significantly reduced compared to that of normal controls (p<0.01). In addition, the expression of activation markers, CD86 on FSC(high) memory B cells from active SLE patients was higher than those of inactive SLE patients and normal controls (p=0.014). Upon stimulation with CpG and IL-15 in vitro for 8 days, isolated FSC(high) memory B cells from active SLE patients revealed augmented production of autoantibodies to chromatin and dsDNA. CONCLUSION: Our results suggest that abnormally activated FSC(high) memory B cells from active SLE patients might be involved in spontaneous production of autoantibodies and induce transition from inactive to active phase of the patients.
