The Expressions of TGF-beta1 and IL-10 in Cultured Fibroblasts after ALA-IPL Photodynamic Treatment.
- Author:
Ji Yeon BYUN
1
;
Ga Youn LEE
;
Hae Young CHOI
;
Ki Bum MYUNG
;
You Won CHOI
Author Information
- Publication Type:Original Article
- Keywords: Aminolevulinic acid; Fibroblasts; IL-10; Photodynamic therapy; TGF-beta1
- MeSH: Acne Vulgaris; Aminolevulinic Acid; Fibroblasts; Granuloma Annulare; Interleukin-10; Interleukins; Lichen Sclerosus et Atrophicus; Light; Photochemotherapy; Psoriasis; RNA, Messenger; Scleroderma, Localized; Skin; Skin Diseases; Transforming Growth Factor beta1; Triazenes
- From:Annals of Dermatology 2011;23(1):19-22
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Topical photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA) was originally used for treating superficial skin tumors. The application of PDT to other inflammatory dermatoses like acne vulgaris, psoriasis, granuloma annulare, localized scleroderma and lichen sclerosus has recently been introduced. However, the underlying mechanisms are not well understood. We've previously reported the induction of tumor growth factor (TGF)-beta1 and interleukin (IL)-10 after PDT with ALA and intense pulsed light (IPL) in cultured HaCaT cells. OBJECTIVE: The purpose of this study was to investigate the expressions of TGF-beta1 and IL-10 in cultured fibroblasts after PDT with using ALA and IPL. METHODS: Cultured fibroblasts were treated with ALA-IPL PDT (1micromol/L of ALA; 0, 4, 8 and 12 J/cm2 of IPL). The expressions of TGF-beta1 and IL-10 were investigated by reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assay. RESULTS: TGF-beta1 mRNA and protein were reduced down to 0.52- and 0.63-fold, respectively, after PDT and the IL-10 protein was increased up to 2.74-fold after PDT. CONCLUSION: The reduction of TGF-beta1 was prominent after PDT and so an antisclerotic effect can be expected after PDT. The induction of IL-10 may contribute to the anti-inflammatory effect, which explains the therapeutic benefit of PDT for inflammatory dermatoses.
