Glucose-Insulin-Potassium Solution Protects Ventricular Myocytes of Neonatal Rat in an In Vitro Coverslip Ischemia/Reperfusion Model.
10.4070/kcj.2015.45.3.234
- Author:
Woo Jung CHUN
1
;
Deuk Young NAH
;
Jun Ho BAE
;
Jin Wook CHUNG
;
Hyunsook LEE
;
Il Soo MOON
Author Information
1. Division of Cardiology, Department of Internal Medicine, Samsung Changwon Hospital, Sungkyunkwan University, College of Medicine, Changwon, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Myocytes, cardiac;
Glucose-insulin-potassium cardioplegic solution;
GlcNAc;
O-GlcNac transferase
- MeSH:
Animals;
Apoptosis;
Glucose;
Insulin;
Muscle Cells*;
Myocytes, Cardiac;
Rats*;
Rats, Sprague-Dawley;
Reactive Oxygen Species;
Shock;
Transferases
- From:Korean Circulation Journal
2015;45(3):234-241
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND AND OBJECTIVES: The benefit of high glucose-insulin-potassium (GIK) solution in clinical applications is controversial. We established a neonatal rat ventricular myocyte (NRVM) in vitro coverslip ischemia/reperfusion (I/R) model and investigated the effects of GIK solution on suppressing reactive oxygen species (ROS) and upregulating O-GlcNacylation, which protects cells from ischemic injury. MATERIALS AND METHODS: NRVMs were isolated from postnatal day 3-4 Sprague-Dawley rat pups and grown in Dulbecco's modified Eagle's medium containing high glucose (4.5 g/L), fetal bovine serum, and penicillin/streptomycin. The effects of the GIK solution on ROS production, apoptosis, and expression of O-GlcNAc and O-GlcNAc transferase (OGT) were investigated in the coverslip I/R model. RESULTS: Covering the 24-well culture plates for 3 hr with 12 mm diameter coverslips resulted in the appropriate ischemic shock. Glucose and insulin synergistically reduced ROS production, protected NRVM dose-dependently from apoptosis, and altered O-GlcNAc and OGT expression. CONCLUSION: The high GIK solution protected NRVM from I/R injury in vitro by reducing ROS and altering O-GlcNacylation.
