Involvement of Oxidative Stress in Cisplatin-Induced Apoptosis in HeLa Cells.
- Author:
Kyoung Hee KO
1
;
Shin Ho KIM
;
Hae Joong CHO
;
Seong Hwan OH
;
Heung Gon KIM
;
Rae Gil PARK
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Wonkwang University, Iksan, Korea.
- Publication Type:Original Article
- Keywords:
Cisplatin;
HeLa cells;
Apoptosis
- MeSH:
Apoptosis*;
Caspases;
Cisplatin;
Flow Cytometry;
Glutathione;
HeLa Cells*;
Humans;
Oxidants;
Oxidative Stress*;
Reactive Oxygen Species
- From:Korean Journal of Obstetrics and Gynecology
2003;46(12):2410-2416
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To determine whether oxidants are formed as part of the cisplatin-induced apoptotic process, intracellular markers of oxidative stress were examined. METHODS: Apoptotic death of HeLa cells by cisplatin was confirmed by flow cytometry. RESULTS: The pre-treatment with glutathione (GSH) significantly attenuated cisplatin-induced apoptosis through the reduction of reactive oxygen species (ROS) accumulation and diminished caspases-3 and 9 protease activity. Furthermore, z-VAD-fmk, an inhibitor of pan-caspase, effectively inhibited the activation of caspases and prevented apoptosis by cisplatin, although cisplatin-induced ROS generation was not attenuated. CONCLUSION: These data indicate that ROS may play a role as an upstream mediator of caspases. Taken together, our results suggest that oxidative stress mediates cisplatin-induced apoptosis in HeLa cells.
