Effects of alpha-Lipoic Acid on Bone Metabolism in Rats with Low Bone Mass.
10.3803/jkes.2005.20.5.476
- Author:
Jung Min KOH
1
;
Hee Sook LEE
;
Duk Jae KIM
;
Ghi Su KIM
Author Information
1. Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Korea.
- Publication Type:Original Article
- Keywords:
Oxidative stress;
Antioxidants;
alpha-Lipoic acid;
Bone Density;
Osteoporosis;
Rats
- MeSH:
Absorptiometry, Photon;
Alkaline Phosphatase;
Animals;
Antioxidants;
Apoptosis;
Body Weight;
Bone Density;
Bone Diseases, Metabolic;
Bone Resorption;
Diet;
Female;
Femur;
Humans;
Immunoenzyme Techniques;
Metabolism*;
Osteoblasts;
Osteoporosis;
Ovariectomy;
Oxidative Stress;
Plasma;
Rats*;
Rats, Sprague-Dawley;
Spine;
Talc;
Thioctic Acid*
- From:Journal of Korean Society of Endocrinology
2005;20(5):476-487
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Growing evidence has shown a biochemical link between increased oxidative stress and reduced bone density. In our previous study, alpha-lipoic acid (alpha-LA), a thiol antioxidant, suppressed both osteoclastogenesis and bone resorption, and also prevented TNF-alpha-induced apoptosis of osteoblast lineages. The effects of alpha-LA were investigated on bone metabolism in rats with a low bone mass. METHODS: An ovariectomy (OVX) or Talc injection (inflammation-mediated osteopenia, IMO) was performed in 12 week old female Sprague-Dawley rats. Diets containing either 0.3%, 0.5% or 1.0% alpha-LA were administered to the OVX rats for 16 weeks, and to the IMO rats for 21 days. The bone mineral densities (BMD) of the anterior-posterior lumbar spine and total femur were measured using dual-energy X-ray absorptiometry (Hologic QDR 4500-A), with small animal software. The plasma bone specific alkaline phosphatase activity (BSAP) and urinary free deoxypyridinoline concentration (DPD) were determined using enzyme immunoassay methods. RESULTS: The body weights were significantly decreased in the OVX rats on the diets containing 0.3 and 0.5% alpha-LA than in the OVX control. No significant differences in the BMD at either site were noted between rats administered the diets with or without alpha-LA. However, the administration of various doses of alpha-LA noticeably decreased the level of urinary DPD in both the OVX and IMO rats. High doses of alpha-LA (0.5% and/or 1.0%) also decreased the levels of plasma BSAP in both models. CONCLUSION: Although no increase in BMD was demonstrated by the administration of alpha-LA, these results suggest that alpha-LA suppresses the rates of bone turnover in rats with a low bone mass
