Expression of TonEBP by Hypertonic and Hyperosmolar Stress in RGC-5 Cells.
10.3341/jkos.2014.55.8.1195
- Author:
Jong Eun WOO
1
;
Min Young KWON
;
Su Wol CHUNG
;
Je Moon WOO
Author Information
1. Department of Ophthalmology, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, Korea. limbus68@naver.com
- Publication Type:Original Article
- Keywords:
Hyperosmolar stress;
Hypertonic stress;
Retinal ganglion cell death;
TonEBP expression
- MeSH:
Blotting, Western;
Glucose;
Mannitol;
NFATC Transcription Factors;
Osmotic Pressure;
Retinal Ganglion Cells;
RNA;
RNA, Messenger;
Staurosporine
- From:Journal of the Korean Ophthalmological Society
2014;55(8):1195-1201
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: In order to determine whether the Tonicity responsive enhancer binding protein (TonEBP) is expressed by hypertonic and hyperosmolar stress, TonEBP expression was investigated in the retinal ganglion cell (RGC) line, RGC-5 cells. METHODS: After RGC-5 cells were cultured by Staurosporine, TonEBP expression was measured with Western immunoblotting analysis and real-time reverse transcription-polymerase chain reaction in 50 mM NaCl, 100 mM mannitol, 50 mM glucose, or 100 mM glucose at 3, 6, 12, and 24 hours after exposure to each environment. RESULTS: In this study, the protein expression of TonEBP was determined to be statistically significantly checked in 50 mM NaCl after 3, and 6 hours, in 100 mM mannitol after 6 hours, and in 100 mM glucose after 3, and 6 hours. TonEBP messenger Ribonucleic acid (mRNA) expression was determined to be statistically significantly checked in 50 mM NaCl after 3 hours, in 100 mM mannitol after 3, and 24 hours, and in 50 mM glucose after 3, and 24 hours. CONCLUSIONS: These results suggested that TonEBP was expressed by hypertonic and hyperosmolar stress at the protein and mRNA levels. Further studies are nedded to determine the role of TonEBP and the mechanism of expression and regulation of TonEBP.
