Single Nucleotide Polymorphisms of Toll-Like Receptor 7 and Toll-Like Receptor 9 in Hepatitis C Virus Infection Patients from Central China.
10.3349/ymj.2014.55.2.428
- Author:
Xin Su WEI
1
;
Chuan Dong WEI
;
Yong Qing TONG
;
Cheng Liang ZHU
;
Ping An ZHANG
Author Information
1. Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, China. zhangpingan@yahoo.com.cn
- Publication Type:Original Article
- Keywords:
Hepatitis C virus;
single nucleotide polymorphism;
TLR7;
TLR9
- MeSH:
Alleles;
China*;
Confidence Intervals;
Female;
Gene Frequency;
Genotype;
Hepacivirus*;
Hepatitis C*;
Hepatitis C, Chronic;
Hepatitis*;
Humans;
Male;
Methods;
Polymorphism, Single Nucleotide*;
Toll-Like Receptor 7*;
Toll-Like Receptor 9*;
Toll-Like Receptors*
- From:Yonsei Medical Journal
2014;55(2):428-434
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To analyze the correlation of polymorphisms of toll-like receptor 7 (TLR7) (rs179009) and toll-like receptor 9 (TLR9) (rs187084) in hepatitis C virus (HCV) infections in the Han population. MATERIALS AND METHODS: The genotypes of TLR7IVS2-151 in HCV infection were detected by Sanger sequencing using polymerase chain reaction-restriction fragment length polymorphism to determine the TLR9 T-1486C single nucleotide polymorphisms (SNP) for all enrolled patients. RESULTS: We found no significant difference between males with spontaneous clearance of HCV versus those chronically infected [chi2=2.71, p=0.10, odd ratios (OR)=0.58, 95% confidence interval (CI) 0.31-1.11]. However, significant differences were found for the distribution of TLR7 (rs179009) in females (chi2=9.46, p=0.01). In females, a significant difference was also found between chronic hepatitis C and those with spontaneous clearance of HCV in terms of TLR7 IVS2-151G/A allele frequencies (chi2=9.50, p=0.00, OR=0.46, 95% CI 0.28-0.75). In HCV-infected patients, no significant association was found between the frequency of TLR9 genotypes and alleles. CONCLUSION: The site of TLR7 IVS2-151 (rs179009) G/A may be a factor for susceptibility of chronic HCV in the female Han population. TLR9T-1486C (rs18084) SNP may not play a major role in HCV infection. However, individual risk profiles for HCV infection did vary by sex and this relationship should be further investigated.
