The Effect of Iron Limmted Condition on Outer Membrane of Vibrio mimicus.
- Author:
Jin Woo JU
;
Seong A JU
;
Joo An OK
;
Cho Rok JUNG
- Publication Type:Original Article
- MeSH:
Bacteria;
Blotting, Western;
Brain;
Electrophoresis, Polyacrylamide Gel;
Enzyme-Linked Immunosorbent Assay;
Gastroenteritis;
Heart;
Human Body;
Humans;
Iron*;
Membrane Proteins;
Membranes*;
Sarcosine;
Vibrio mimicus*;
Vibrio*
- From:Journal of the Korean Society for Microbiology
1999;34(2):147-155
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Vibrio mimicus, marine bacteria pathogenic for fish, can causes acute gastroenteritis in human. Iron limmited condition like in human body, may change the surface structure of V. mimicus. In this study we obse'rved the effect of iron limmited condition on outer membrane protein of V. mimicus. Ethylenediamine-di (O-hydroxy-phenylacetic) acid (EDDA), an iron chelator, delayed the time to reach expotential growth of V. mimicus in brain heart infusion medium from 3 hours to 20 hours. Outer membrane protein of V. mimicus-CON (cultured in BHI) and V. mimicus-EDDA (cultured in BHI contain EDDA) were seperated by 1% sarcosine from total cell envelop. SDS-PAGE of V. mimicus-EDDA and V. mimicus-CON showed similar protein profiles contain 37 kDa major protein but 86 and 90 kDa protein were induced differently. Immunological properties of above protein were determined by ELISA and western blotting. 86 kDa EDDA- specific OMP was induced in V. mimicus (isolate 96-1), V. parahaemolyticus (serotype 09), V. alginolyticus (isolate 95-1), E. coli (human isolate) and V. vulnificus ATCC 27562 in iron limmited condition.
