Genotyping of ABO and D Antigens.
- Author:
Heung Bum OH
1
;
Youn Jung CHO
;
Young Hee CHO
;
Yoo Sung HWANG
;
Doo Sung KIM
;
Sang In KIM
Author Information
1. Research Institute for Blood Transfusion, The Republic of Korea National Red Cross, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
ABO genotyping;
D genotyping;
PCR;
RFLP
- MeSH:
Alleles;
Blood Donors;
DNA Fingerprinting;
Gene Amplification;
Genotype;
Humans;
Paternity;
Phenotype;
Polymerase Chain Reaction;
Polymorphism, Restriction Fragment Length
- From:Korean Journal of Blood Transfusion
1997;8(1):31-37
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Genes for ABO and Rh phenotypes were recently identified. Although ABO genotyping don't find wide application in hospital transfusion services, it can play an important role in paternity and forensic investigation. In case of Rh system, however, DNA typing may find several practical applications such as prenatal determination of fetal Rho(D) genotype. METHODS: 64 blood samples for ABO genotyping were collected from blood donors (34 A, 30 B) and 18 samples for D genotyping (10 D+, 8 D-). To distinguish A, B and O alleles, we analyzed nucleotide positions 261 and 803 using polymerase chain reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP). PCR products containing nucleotide position 261 were restricted with KpnI and BstEII. Rh genotyping was done by two sets of primers, one set for both RhD and RhCcEe gene amplification, and the other set for RhD only. RESULTS: The frequencies of ABO genotypes found in Korean blood donors were as follows: in the phenotype A group, AO=79% and AA= 21%; and in the phenotype B group, BO=93% and BB=7%. Of 18 blood samples for D genotytping, 10 were typed as RhD positive and 8 as RhD negative, showing full agreement with serological typing. CONCLUSION: ABO and D genotyping can be used when RBCs suitable for serological phenotyping are not available. Futhermore, these will be useful as a supplemental test to solve the problem of blood group typing caused by weak ABO and Rh phenotype.
