Changes of Renal Calcium Content and CaOx Crystal Deposit after Unilateral Nephrectomy in Rats.
- Author:
Myung Seung PARK
1
;
Young Gon KIM
Author Information
1. Department of Urology, Chonbuk National University Medical School, Chonju, Korea.
- Publication Type:Original Article
- Keywords:
Unilateral nephrectomy;
Renal calcium content
- MeSH:
Absorption;
Adult;
Ammonium Chloride;
Animals;
Calcium Oxalate;
Calcium*;
Citric Acid;
Creatinine;
Diet;
Drinking Water;
Ethylene Glycol;
Humans;
Hydrogen-Ion Concentration;
Kidney;
Magnesium;
Male;
Models, Animal;
Nephrectomy*;
Phosphorus;
Potassium;
Rats*;
Sodium;
Uric Acid;
Urinary Calculi;
Water
- From:Korean Journal of Urology
1998;39(12):1161-1165
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The surgical removal of normal kidney elicits dramatic changes in the remaining kidney. Changes in urinary excretion of lithogenlc substances and urinary inhibitor may increase or decrease the stone-forming potential. The aim of this study was to determine if unilateral nephrectomy could enhance urinary calculi formation in animal model. MATERIALS AND METHODS: Adult male rats were divided randomly into 3 groups. Group 1(n=5) were fed with pellet and tap water for 4 weeks without nephrectomy Group 2(n=5) were fed with the same pellet and 0.8% ethylene glycol plus 1% ammonium chloride in drinking water for 4 weeks without nephrectomy Group 3(n=5) were fed with the same pellet and the same lithogenic diet from 2 weeks after uninephrectomy for 4 weeks. Each right kidney was harvested after 4 weeks feeding. One half of the each kidney were fixed for H & E stain to examine crystal deposits. The remaining half of the kidneys were treated with 12N KOH and 6N HCI for the measurement of calcium content with atomic absorption spectrophotometer. About 3-5cc of blood was collected from the inferior vela cava for determinations of serum creatinine, calcium, magnesium and phosphorus & chloride. Urine was collected over a 24 hour interval before and after lithogenic diet, and urine volume, 24-hour urinary excretions of oxalate, citrate, uric acid, calcium, magnesium, phosphate, sodium, potassium, chloride, and creatinine were measured. RESULTS: There was a significant difference between group 1(0.243 +/- 0.014mmo1/kg) and lithogenic diet groups(group 2 and group 3 in calcium content(p < 0.01)). But, there was no significant difference of calcium content between group 2(5.475 +/- 0.247mmo1/kg) and group 3(5.843 +/- 0.324mmol/kg)(p > 0.05). There were no calcium oxalate crystal deposit in the group 1, but there were about 2-5 crystal deposits with partially polarized light microscope( x 100) in group 2 and group 3, and there was no significant difference between group 2 and group 3. After taking lithogenic diet, the serum creatinine, serum magnesium, serum phosphors, urine pH & 24-hour urinary excretions of oxalate increased significantly in group 2 and group 3, whereas creatinine clearance & 24-hour urinary excretions of calcium, sodium, potassium, chloride, citrate decreased significantly in the same groups(p < 0.05). But, there were no significant difference of serum calcium, chloride, and 24-hour urinary excretions of magnesium, uric acid among three groups after 4 weeks feeding. CONCLUSIONS: Unilateral nephrectomy would not increase stone forming potential.
