Analysis of Cytokines in Sera from Type 1 Diabetic Patients at Diagnosis.
10.6065/jkspe.2011.16.1.13
- Author:
In Suk YOON
1
;
Choong Ho SHIN
;
Sei Won YANG
Author Information
1. Department of Pediatrics, Gundan Top Hospital, Incheon, Korea.
- Publication Type:Original Article
- Keywords:
Diabetes mellitus, type 1;
Cytokines;
Interleukin-1beta;
Tumor necrosis factor-alpha;
Interleukin-10;
Islet cell antibody
- MeSH:
Autoantibodies;
Cytokines;
Diabetes Mellitus, Type 1;
Humans;
Insulin;
Interleukin 1 Receptor Antagonist Protein;
Interleukin-10;
Interleukin-12;
Interleukin-1beta;
Interleukin-2;
Interleukin-4;
Interleukin-6;
Tumor Necrosis Factor-alpha
- From:Journal of Korean Society of Pediatric Endocrinology
2011;16(1):13-19
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Diverse cytokines influence the pathogenesis of type 1 diabetes mellitus (T1DM) in different ways. We studied the profile of cytokines in sera from type 1 diabetic patients at diagnosis. METHODS: Serum levels of 11 cytokines (IL-1alpha, IL-1beta, IL-1Ra, IL-2, IL-4, IL-6, IL-10, IL-12 (p70), INF-gamma, and TNF-alpha) from 38 newly-diagnosed T1DM patents and 39 healthy controls were measured, using multiplex immunoanalytic xMAP. RESULTS: Patients showed significantly higher levels of IL-1beta (P < 0.01), IL-10 (P < 0.01), and TNF-alpha (P = 0.019), than the healthy controls. In 12 of 35 patients, the insulin autoantibody (IAA) was positive (34%) and the level of IAA was correlated with IL-10 (r = 0.454, P = 0.006), and TNF-alpha (r = 0.368, P = 0.030). CONCLUSION: These results suggest that IL-1beta, TNF-alpha, and IL-10 play a role in the pathogenesis of T1DM, and the level of the IAA is correlated with IL-10 and TNF-alpha.
