Matrix metalloproteinase-8 and substance P levels in root canal exudates of nonvital teeth.
10.5395/JKACD.2011.36.3.196
- Author:
Su Jung SHIN
1
;
Woocheol LEE
;
Jae Il LEE
;
Seung Ho BAEK
;
Kee Yeon KUM
;
Won Jun SHON
;
Kwang Shik BAE
Author Information
1. Department of Conservative Dentistry, College of Dentistry, Yonsei University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Canal exudate;
MMP-8;
SP;
Pain
- MeSH:
Dental Pulp Cavity;
Enzyme-Linked Immunosorbent Assay;
Ethics Committees, Research;
Exudates and Transudates;
Humans;
Inflammation;
Informed Consent;
Matrix Metalloproteinase 8;
Percussion;
Prospective Studies;
Substance P;
Tooth;
Tooth, Nonvital
- From:Journal of Korean Academy of Conservative Dentistry
2011;36(3):196-202
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: The aim of this study was to investigate levels of matrix metalloproteinase-8 (MMP-8) and substance P (SP) in root canal exudates during root canal treatment (RCT) of nonvital, painful teeth. MATERIALS AND METHODS: Patients scheduled for nonsurgical RCT were prospectively selected; the study was performed after obtaining informed consent from the patients and was approved by the Institutional Review Board for Clinical Research of Gangnam Severance Hospital, Yonsei University (3-2008-0118). Canal exudates samples were collected using sterilized paper points from teeth scheduled for RCT across three different time periods. MMP-8 and SP levels were measured using enzyme-linked immunosorbent assay (ELISA). Data were analyzed using a mixed model analysis and the Pearson correlation analysis (p < 0.05). RESULTS: MMP-8 and SP levels in GCF were decreased during RCT (p < 0.0001), and they showed a weak positive correlation to each other (p < 0.05). Patients'subjective pain levels and the response from percussion test were significantly related to SP level. CONCLUSIONS: This study demonstrated that periradicular inflammation endodontic origin can elevate SP and MMP-8 levels in root canal exudates. Interestingly, SP level of canal exudates showed a possibility of being used as an indicator of pain due to periapical pathosis.
