Epidemiological Surveillance of P and G Genotypes of Group A Rotavirus Detected from Diarrheic Patients in Daejeon Region.
10.4167/jbv.2007.37.3.169
- Author:
Sun Kyoung PARK
1
;
Young Sun HEO
;
Sun Hye BING
;
Doo Sung CHEON
;
Jiaqi CHU
;
Moo Hyung JUN
Author Information
1. Daejeon Metropolitan City Institute of Health & Environment, Daejeon, Korea Center for Disease Control, Seoul, Republic of Korea.
- Publication Type:Original Article
- Keywords:
Rotavirus;
Acute gastroenteritis;
P and G genotypes;
Nested multiplex PCR
- MeSH:
Diarrhea;
Enzyme-Linked Immunosorbent Assay;
Epidemiologic Studies;
Gastroenteritis;
Genotype*;
Humans;
Multiplex Polymerase Chain Reaction;
Polymerase Chain Reaction;
Reverse Transcription;
Rotavirus*
- From:Journal of Bacteriology and Virology
2007;37(3):169-175
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
During 3 years surveillance (January 2001 through December 2003) for acute gastroenteritis in human in Daejeon region, 432 out of 4,869 stool samples were selected as rotavirus-positive specimens by means of antigen-capture enzyme-linked immunosorbent assay (ELISA). The P (VP4) and G (VP7) genotypes for 432 stool samples were investigated by reverse transcription polymerase chain reaction (RT-PCR) and nested multiplex PCR. The most prevalent P subtype was P[8] (44.9%), followed by P[4] (25.7%) and P[6] (17.1%). No cases for P[10] and P[9] subtypes were found through the study. In G subtyping, G1 (53.2%) was the most frequently found G type, followed by G2 (23.1%), G3 (9.5%), G4 (6.7%), and G9 (0.9%). The order of detection rates for G2, G3 and G4 was variable by years. The most common G- and P- type combination found in this study was G1P[8] (33.1%), followed by G2P[4] (20.4%), G1P[6] (10.0%), G3P[8] (7.2%) and G4P[6] (4.2%). The mixed types of G and P were observed most frequently in P[8] (1.4%) and G1 (3.2%), respectively. This is the first molecular epidemiological study for Group A rotavirus in Daejeon region. The results might be useful data for evaluating the epidemiological status of rotaviral diarrhea in the region.
