5' and 3' cis-Acting RNA Elements Required for RNA Replication of Porcine Reproductive and Respiratory Syndrome Virus.
10.4167/jbv.2007.37.3.193
- Author:
Shien Young KANG
1
;
Yu Jeong CHOI
;
Sang Im YUN
;
Byung Hak SONG
;
Young Min LEE
Author Information
1. Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea.
- Publication Type:Original Article
- Keywords:
Porcine reproductive and respiratory syndrome virus;
Arterivirus;
RNA replication;
cis-acting elements
- MeSH:
Arteriviridae;
Arterivirus;
DNA, Complementary;
Genome;
Humans;
Mental Competency;
Nucleotides;
Polyadenylation;
Porcine Reproductive and Respiratory Syndrome*;
Porcine respiratory and reproductive syndrome virus*;
RNA*;
Swine
- From:Journal of Bacteriology and Virology
2007;37(3):193-201
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Porcine reproductive and respiratory syndrome virus (PRRSV), a member of the genus Arterivirus in the family Arteriviridae, is the most important viral pathogens in swine industry worldwide. Here, we have investigated 5' and 3' cis-acting RNA elements required for PRRSV genome replication. Using the infectious PRRSV cDNA, we have manipulated the genomic RNA to generate mutant genomic RNAs, transfected these mutants into susceptible MARC-145 cells, and examined the competence of RNA replication. We found three genetic factors that were essential for viral replication. First, the cap structure present at the 5'-end of the genome was absolutely required for RNA replication. Secondly, polyadenylation of the genomic RNA at the 3'-end was also essential for RNA replication. Thirdly, approximately 100-nucleotide region just upstream of the N protein-coding region was crucial for genomic RNA replication. Taken together, our findings indicate that replication of PRRSV genomic RNA requires three important cis-acting RNA elements: 5' cap structure, 3' poly(A) motif, and an internal sequence of about 100 nucleotides. Further investigation is needed to elucidate the molecular mechanism(s) of how these elements act on PRRSV genome replication.
