B Cells Transduced with HPV16 E6/E7-expressing Adenoviral Vector Can Efficiently Induce CTL-dependent Anti-Tumor Immunity.
- Author:
Yun Sun KIM
1
;
Hyun Jeong KO
;
Yeon Jeong KIM
;
Seung Hee HAN
;
Jung Mi LEE
;
Woo Sung CHANG
;
Hyun Tak JIN
;
Young Chul SUNG
;
Chang Yuil KANG
Author Information
- Publication Type:Original Article
- Keywords: HPV16 E6/E7; adenoviral vector; B cells; anti-tumor immunity
- MeSH: Adenoviridae; B-Lymphocytes*; Dendritic Cells; Female; Humans; Immune System; Immunotherapy; Oncogene Proteins; Uterine Cervical Neoplasms; Vaccination; Vaccines
- From:Immune Network 2007;7(3):109-116
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Human papillomavirus (HPV) infection is responsible for cervical cancer, a common cancer in women. Since HPV infection and cancer development are controlled by the host immune system, immunotherapy against HPV can be helpful in preventing or treating HPV-associated cervical cancer. Two oncoproteins of HPV16, E6 and E7, are promising targets for immunotherapy against cervical cancer, because they are constitutively expressed in cervical cancer. METHODS: Since cellular vaccines using B cells as well as dendritic cells offer an efficient approach to cancer immunotherapy, we opted to use B cells. We evaluated the immunogenicity and anti-tumor effects of a B cell vaccine transduced with HPV16 E6/E7-expressing adenovirus. RESULTS: Vaccination with HPV16 E6/E7-transduced B cells induced E6/E7-specific CD8+ T cell-dependent immune responses and generated anti-tumor effects against E6/E7-expressing TC-1 tumor. The anti-tumor effect induced by this B cell vaccine was similar to that elicited by DC vaccine, showing that B cells can be used as an alternative to dendritic cells for cellular vaccines. CONCLUSION: Thisstudy has shown the feasibility of using B cells as immunogenic APCs and the potential for developing prophylactic and therapeutic vaccines against HPV-associated cervical cancer using a B cell vaccine transduced with adenovirus expressing HPV16 E6/E7.
