Tumor Necrosis Factor and Lymphotoxin-alpha Gene Polymorphism in Korean Children with Type 1 Diabetes.
- Author:
Jin Soon SUH
1
;
So Young PARK
;
Min Ho JUNG
;
Byung Kyu SUH
;
Tae Gyu KIM
;
Byung Churl LEE
Author Information
1. Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea. byungcl@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Type 1 diabetes;
Gene;
Polymorphism;
Tumor necrosis factor;
Lymphotoxin-alpha
- MeSH:
Asian Continental Ancestry Group;
Child*;
Diabetic Ketoacidosis;
Diagnosis;
Disease Susceptibility;
Female;
Genotype;
Humans;
Lymphotoxin-alpha*;
Polymerase Chain Reaction;
Promoter Regions, Genetic;
Tumor Necrosis Factor-alpha*
- From:Korean Journal of Pediatrics
2005;48(8):871-876
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Recently, it was reported that tumor necrosis factor (TNF) and lymphotoxin-alpha (LT-alpha) gene regions might be a susceptible loci to type 1 diabetes in Japanese. The purpose of this study was to investigate the association of TNF and LT-alpha gene polymorphisms with disease susceptibility in Korean children with type 1 diabetes. METHODS: Forty-nine Korean children with type 1 diabetes (29 girls and 20 boys) and 94 healthy Koreans were investigated in this study. Genotyping for -857T/C polymorphism in the TNF promoter region and LT-alpha gene polymorphism were performed by PCR-RFLP (restriction fragment length polymorphism). TNF promoter -1031C/T polymorphism was detected by allele-specific PCR. RESULTS: The distribution of the -857T/C and -1031C/T genotype in the TNF promoter region was not different between diabetic children and the controls. The frequency of TT genotype in the distribution of TNF -1031C/T polymorphism in diabetic children with diabetic ketoacidosis (DKA) at diagnosis was significantly lower than those without DKA (P< 0.05). No significant difference in the distribution of LT-alpha gene polymorphism was observed between diabetic children and the controls. There was no association between clinical characteristics of type 1 diabetes and LT-alpha gene polymorphisms. CONCLUSION: These results suggest that TNF promoter -857T/C and LT-alpha gene polymorphisms are not associated with susceptibility to type 1 diabetes in Korean children. TNF promoter -1031C/T polymorphism might be related to clinical manifestations (DKA) of type 1 diabetes.
