Effects of Hyperosmolar Stimuli on Activation of Human Eosinophilic Leukaemia EoL-1 Cells.
- Author:
Byoung Chul KWON
1
;
Eun Soo KIM
;
Kyung Won KIM
;
Tae Won SONG
;
Myung Hyun SOHN
;
Myeong Heon SHIN
;
Kyu Earn KIM
Author Information
1. Department of Pediatrics, Yonsei University College of Medicine, Seoul, Korea. kekim@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Exercise-induced asthma;
Hyperosmolar;
EoL-1 cells
- MeSH:
Asthma, Exercise-Induced;
Bronchoconstriction;
Cell Line;
Dehydration;
Eosinophils*;
Humans*;
Intercellular Adhesion Molecule-1;
Superoxides
- From:Korean Journal of Pediatrics
2005;48(8):881-885
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Airway dehydration and subsequent hyperosmolarity of periciliary fluid are considered critical events in exercise-induced bronchoconstriction. The aim of this study was to establish if a hyperosmolar challenge could induce activation of eosinophils. METHODS: Human eosinophilic leukaemic cell lines, EoL-1 cells were incubated with hyperosmolar solutions for 15 minutes. Activation of EoL-1 cells was monitored by degranulation and superoxide anion production. In addition, we examined surface expression of CD69 and ICAM-1. RESULTS: Hyperosmolar stimuli didn't induce superoxide anion production and degranulation. In addition, EoL-1 cells cultured with hyperosmolar medium at 930 mOsm/kg H2O resulted in no significant increment in fluorescent intensity of CD69 and ICAM-1 expression compared with results for cells incubated with isomolar medium. CONCLUSION: We found that hyperosmolar stimuli don't cause activation of EoL-1 cells, but further studies are required to determine the role of eosinophil in the mechanism of exercise-induced asthma.
