Inactivated pep27 mutant as an effective mucosal vaccine against a secondary lethal pneumococcal challenge in mice.
- Author:
Sang Yoon CHOI
1
;
Thao Dang Hien TRAN
;
David E BRILES
;
Dong Kwon RHEE
Author Information
- Publication Type:Original Article
- Keywords: Streptococcus pneumoniae; pep27 mutant; Inactivated vaccine; No antibiotic resistance marker; Mucosal immunity
- MeSH: Animals; Colon; Immune Sera; Immunity, Mucosal; Immunization; Mice; Sprains and Strains; Streptococcus pneumoniae
- From:Clinical and Experimental Vaccine Research 2013;2(1):58-65
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: A pep27 mutant may be able to elicit mucosal immunity against pneumococcal diseases, and could be employed as an inexpensive attenuated vaccine. However, this particular mutant contains an erythromycin-resistance marker. The purpose of the current study is to develop a markerless pep27 mutant and assess whether this inactivated mutant is able to induce mucosal immunity. MATERIALS AND METHODS: Mice were vaccinated intranasally with the inactivated markerless pep27 mutant every 2 weeks for a total of three times, after which time serum samples were analyzed for antibody titers. The mice were then challenged with a lethal D39 strain and their survival time was measured. The cross-reactivity of the antisera against pep27 was also compared to other mutant serotypes. RESULTS: Intranasal immunization of mice with the inactivated markerless pep27 mutant provides effective protection and rapidly cleared bacterial colonization in vivo. Moreover, antisera raised against the pep27 mutant may cross-react with several other serotype strains. CONCLUSION: Intranasal immunization with the inactivated pep27 mutant may be able to provide mucosal immunity, and could represent an efficient mucosal vaccine.
