Alteration of Molecules and Cytokines Related to the Activation of T Lymphocyte in Immune Tolerance Induced Mice Model.
- Author:
Sung Mo HUR
1
;
Choon Hyuck KWON
;
Jae Won JOH
;
Sung Joo KIM
Author Information
1. Department of Surgery, Sungkyunkwan University School of Medicine, Seoul, Korea. kmhyj@smc.samsung.co.kr
- Publication Type:Original Article
- Keywords:
CD45RB;
Monoclonal antibody;
T cell;
Allogenic skin transplantation
- MeSH:
Abatacept;
Allografts;
Animals;
Antibodies, Monoclonal;
Bone Marrow;
Busulfan;
Cytokines*;
Enzyme-Linked Immunosorbent Assay;
Flow Cytometry;
Graft Survival;
Humans;
Immune Tolerance*;
Lymphocytes*;
Mice*;
Models, Animal;
Skin;
Skin Transplantation;
T-Lymphocytes;
Tissue Donors;
Transplants
- From:The Journal of the Korean Society for Transplantation
2005;19(2):119-123
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Monoclonal antibodies (mAb) specific for CD45RB as a potent tolerogenic target can prolong allograft survival in several animal models. The mechanisms of CD45RB mAb-mediated tolerance are largely unknown. Therefore, the present studies were performed to determine the immunomodulatory effects of CD45RB mAb on T cells in early or late time after allogenic skin transplantation. METHODS: Skin grafts and bone marrows from BALB/c donor mice were transplanted on C57BL/6 recipient mice and Busulfan was administerd. Group 1 was composed of anti-CD154 mAb administerd mice, group 2 was composed of anti-CD154 and anti-CD45RBB mAb administerd mice, and group 3 consisted of anti-CD154 mAb and CTLA4-Ig administerd mice. The proportion of splenic CD4+ and CD8+ T cell and range of CD45RB was observed by flow cytometry. Cytokines secreted by CD4+ T cell were measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: CD45RB mAb in combination with CD154 mAb enhanced graft survival in allogenic skin transplantation model where CD45RB mAb specific for CD45RB, which was proven mainly expressed by CD8+ T cells, had inhibitory effects on the proportion of splenocyte-derived CD8+ and CD4+CD45RB(high) T cells in early or late time posttransplant. CONCLUSION: The combined therapy showed decreases in the proliferation of CD8+ T cells in vivo and allospecific responses of IFN-gamma-producing cells. Such immunomodulatory effects may be associated with the tolerogenic ability of CD45RB mAb in allogenic skin transplantation.
