The Effect of Vasoactive Intestinal Peptide on Cord Blood CD34 (+) Cells.
- Author:
Mi Kyong YEO
1
;
Hwa Young BAE
;
Min Kyu HUR
;
Jae Sun RA
;
In Seob HAN
;
Min Keong KIM
;
Soon Ki KIM
;
Sang Kyu PARK
Author Information
1. Department of Pediatrics, Ulsan University Hospital, Korea. sang@uuh.ulsan.kr
- Publication Type:Original Article
- Keywords:
Vasoactive intestinal peptide;
Vasoactive intestinal peptide receptor;
Umbilical cord blood;
CD34 cells;
CFU-GEMM;
CFU-Mk
- MeSH:
Clinical Coding;
Erythrocytes;
Fetal Blood*;
Humans;
Megakaryocyte Progenitor Cells;
Megakaryocytes;
Myeloid Progenitor Cells;
Neuropeptides;
Receptors, Vasoactive Intestinal Peptide;
RNA;
Stem Cells;
Vasoactive Intestinal Peptide*
- From:Korean Journal of Pediatric Hematology-Oncology
2003;10(2):262-268
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We investigated the expression of vasoactive intestinal peptide (VIP), VIP receptor 1 (VPAC1), VIP receptor 2 (VPAC2) genes in the human umbilical cord blood CD34 cells, and the ability of VIP to stimulate human primitive as well as monopotent hematopoietic progenitors. METHODS: We isolated RNA from umbilical cord blood CD34 cells, and then performed RT-PCR, and sequencing. The umbilical cord blood CD34 cells were cultured with the various concentrations of VIP for burst-forming unit of erythrocyte (BFU-E), colony-forming unit of granulocyte/monocyte (CFU-GM), colony-forming unit of graulocyte/erythrocyte/monocyte/megakaryocyte (CFU-GEMM), and colony-forming unit of megakaryocyte (CFU-Mk). RESULTS: The RNA coding for VPAC1 was detected in human umbilical cord blood CD34 cells. VIP significantly stimulated the growth of CFU-GEMM and CFU-Mk. CONCLUSION: The present results suggest that VIP is an important neuropeptide in the early proliferation of human primitive as well as megakaryocyte progenitors.
