Antepartal insulin-like growth factor concentrations indicating differences in the metabolic adaptive capacity of dairy cows.
10.4142/jvs.2014.15.3.343
- Author:
Marion PIECHOTTA
1
;
Lars HOLZHAUSEN
;
Marcelo Gil ARAUJO
;
Maike HEPPELMANN
;
Anja SIPKA
;
Chistiane PFARRER
;
Hans Joachim SCHUBERTH
;
Heinrich BOLLWEIN
Author Information
1. Clinic for Cattle, University of Veterinary Medicine, 30173 Hannover, Germany. marion.piechotta@tiho-hannover.de
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
cattle;
growth hormone;
insulin-like growth factor I;
metabolism;
suppressor of cytokine signaling
- MeSH:
Animals;
Cattle;
Estradiol/blood;
Female;
Growth Hormone/blood;
Insulin/blood;
Insulin-Like Growth Factor Binding Protein 2/analysis;
Insulin-Like Growth Factor Binding Protein 3/analysis;
Insulin-Like Growth Factor Binding Protein 4/analysis;
Insulin-Like Growth Factor I/*analysis/physiology;
Liver/chemistry;
Pregnancy/metabolism/physiology;
Pregnancy, Animal/*metabolism/physiology;
Progesterone/blood;
Suppressor of Cytokine Signaling Proteins/analysis;
Thyroid Hormones/blood
- From:Journal of Veterinary Science
2014;15(3):343-352
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cows with different Insulin-like Growth Factor-I (IGF-I) concentrations showed comparable expression levels of hepatic growth hormone receptor (GHR). Suppressor of cytokine signaling 2 (SOCS2), could be responsible for additional inhibition of the GHR signal cascade. The aims were to monitor cows with high or low antepartal IGF-I concentrations (IGF-I(high) or IGF-I(low)), evaluate the interrelationships of endocrine endpoints, and measure hepatic SOCS2 expression. Dairy cows (n = 20) were selected (240 to 254 days after artificial insemination (AI)). Blood samples were drawn daily (day -17 until calving) and IGF-I, GH, insulin, thyroid hormones, estradiol, and progesterone concentrations were measured. Liver biopsies were taken (day 264 +/- 1 after AI and postpartum) to measure mRNA expression (IGF-I, IGFBP-2, IGFBP-3, IGFBP-4, acid labile subunit (ALS), SOCS2, deiodinase1, GHR1A). IGF-I concentrations in the two groups were different (p < 0.0001). However, GH concentrations and GHR1A mRNA expression were comparable (p > 0.05). Thyroxine levels and ALS expression were higher in the IGF-I(high) cows compared to IGF-I(low) cows. Estradiol concentration tended to be greater in the IGF-I(low) group (p = 0.06). It was hypothesized that low IGF-I levels are associated with enhanced SOCS2 expression although this could not be decisively confirmed by the present study.
