Kinetin inhibits apoptosis of aging spleen cells induced by D-galactose in rats.
10.4142/jvs.2014.15.3.353
- Author:
Mengyun LI
1
;
Wuqing OUYANG
;
Xiaoli WU
;
Yin ZHENG
;
Yunpeng WEI
;
Lei AN
Author Information
1. College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China. oywq2014@163.com
- Publication Type:Original Article
- Keywords:
apoptosis;
immunity;
kinetin;
mitochondrial membrane potential;
spleen
- MeSH:
Aging/drug effects/physiology;
Animals;
Apoptosis/drug effects/*physiology;
Female;
Galactose/*pharmacology;
Interleukin-6/physiology;
Interleukins/physiology;
Kinetin/pharmacology/*physiology;
Male;
Membrane Potential, Mitochondrial/drug effects/physiology;
Rats;
Spleen/*cytology/drug effects/physiology
- From:Journal of Veterinary Science
2014;15(3):353-359
- CountryRepublic of Korea
- Language:English
-
Abstract:
Kinetin (Kn) is a cytokinin growth factor that exerts several anti-aging and antioxidant effects on cells and organs. To investigate the mechanism underlying apoptotic events in aging cells induced by D-galactose (D-gal), we examined the effect of Kn delivered via nuchal subcutaneous injection on D-gal-induced aging and apoptosis in rats. Our results showed that interleukin (IL)-2 levels and mitochondrial membrane potential (DeltaPsim) were decreased by Kn in aging rats while IL-6 production and apoptosis increased. In addition, the expression of anti-apoptotic Bcl-2 was low while that of Bax was high in the aging group. After treated with Kn, compared with aging group, there showed obvious difference in Kn group with elevated IL-2, proliferation index, Bcl-2, DeltaPsim and decreased IL-6 and Bax in splenic lymphocyte. Based on these results, we concluded that Kn can effectively protect the rat spleen from aging, apoptosis, and atrophy.
