Molecular characterization of duck enteritis virus CHv strain UL49.5 protein and its colocalization with glycoprotein M.
10.4142/jvs.2014.15.3.389
- Author:
Meng LIN
1
;
Renyong JIA
;
Mingshu WANG
;
Xinghong GAO
;
Dekang ZHU
;
Shun CHEN
;
Mafeng LIU
;
Zhongqiong YIN
;
Yin WANG
;
Xiaoyue CHEN
;
Anchun CHENG
Author Information
1. Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, China. jiary@sicau.edu.cn, chenganchun@vip.163.com
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
colocalization;
duck enteritis virus;
intracellular localization;
molecular characterization;
UL49.5 protein
- MeSH:
Animals;
Ducks/virology;
Genes, Viral/genetics;
Mardivirus/*genetics;
Membrane Glycoproteins/*genetics;
Microscopy, Fluorescence;
Phylogeny;
Polymerase Chain Reaction/veterinary;
Viral Envelope Proteins/*genetics
- From:Journal of Veterinary Science
2014;15(3):389-398
- CountryRepublic of Korea
- Language:English
-
Abstract:
The UL49.5 gene of most herpesviruses is conserved and encodes glycoprotein N. However, the UL49.5 protein of duck enteritis virus (DEV) (pUL49.5) has not been reported. In the current study, the DEV pUL49.5 gene was first subjected to molecular characterization. To verify the predicted intracellular localization of gene expression, the recombinant plasmid pEGFP-C1/pUL49.5 was constructed and used to transfect duck embryo fibroblasts. Next, the recombinant plasmid pDsRed1-N1/glycoprotein M (gM) was produced and used for co-transfection with the pEGFP-C1/pUL49.5 plasmid to determine whether DEV pUL49.5 and gM (a conserved protein in herpesviruses) colocalize. DEV pUL49.5 was thought to be an envelope glycoprotein with a signal peptide and two transmembrane domains. This protein was also predicted to localize in the cytoplasm and endoplasmic reticulum with a probability of 66.7%. Images taken by a fluorescence microscope at different time points revealed that the DEV pUL49.5 and gM proteins were both expressed in the cytoplasm. Overlap of the two different fluorescence signals appeared 12 h after transfection and continued to persist until the end of the experiment. These data indicate a possible interaction between DEV pUL49.5 and gM.
