Enhancing mucosal immunity in mice by recombinant adenovirus expressing major epitopes of porcine circovirus-2 capsid protein delivered with cytosine-phosphate-guanosine oligodeoxynucleotides.
10.4142/jvs.2014.15.3.399
- Author:
Hong Tao CHANG
1
;
Xiu Yuan HE
;
Yu Feng LIU
;
Lu CHEN
;
Quan Hai GUO
;
Qiu Ying YU
;
Jun ZHAO
;
Xin Wei WANG
;
Xia YANG
;
Chuan Qing WANG
Author Information
1. Animal Infectious Disease Lab, College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China. chenluhau@hotmail.com
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
cytosine-phosphate-guanosine oligodeoxynucleotides;
mucosal immunity;
porcine circovirus-2;
rAd/Cap/518
- MeSH:
Adenoviridae/genetics/immunology;
Administration, Intranasal;
Animals;
Capsid Proteins/*genetics/immunology;
Circoviridae Infections/*immunology;
Circovirus/*genetics/immunology;
Epitopes/genetics/immunology;
Female;
Immunity, Mucosal/immunology;
Immunoglobulin A/blood/immunology;
Immunoglobulin G/blood/immunology;
Mice;
Mice, Inbred BALB C;
Oligodeoxyribonucleotides/genetics;
Vaccines, Synthetic/genetics/immunology;
Viral Vaccines/administration & dosage/*genetics/immunology
- From:Journal of Veterinary Science
2014;15(3):399-407
- CountryRepublic of Korea
- Language:English
-
Abstract:
A recombinant replication-defective adenovirus expressing the major epitopes of porcine circovirus-2 (PCV-2) capsid protein (rAd/Cap/518) was previously constructed and shown to induce mucosal immunity in mice following intranasal delivery. In the present study, immune responses induced by intranasal immunization with a combination of rAd/Cap/518 and cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODN) were evaluated in mice. The levels of PCV-2-specific IgG in serum and IgA in saliva, lung, and intestinal fluids were significantly higher in the group immunized with rAd/Cap/518 and CpG ODN than animals immunized with rAd/Cap/518 alone. The frequencies of IL-2-secreting CD4+ T cells and IFN-gamma-producing CD8+ T cells were significantly higher in the combined immunization group than mice immunized with rAd/Cap/518 alone. The frequencies of CD3+, CD3+CD4+CD8-, and CD3+CD4-CD8+ T cells in the combined immunization group were similar to that treated with CpG ODN alone, but significantly higher than mice that did not receive CpG ODN. PCV-2 load after challenge in the combined immunization group was significantly lower than that in the phosphate-buffered saline placebo group and approximately 7-fold lower in the group treated with CpG ODN alone. These results indicate that rAd/Cap/518 combined with CpG ODN can enhance systemic and local mucosal immunity in mice, and represent a promising synergetic mucosal vaccine against PCV-2.
