A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C.
10.4142/jvs.2014.15.3.423
- Author:
Yao Zhong DING
1
;
Jian Hua ZHOU
;
Li Na MA
;
Yan Ni QI
;
Gang WEI
;
Jie ZHANG
;
Yong Guang ZHANG
Author Information
1. State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. zhangjie03@caas.cn
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
foot-and-mouth disease;
foot-and-mouth disease virus serotype C;
reverse transcription loop-mediated isothermal amplification;
reverse transcription-PCR, sensitivity
- MeSH:
Animals;
Foot-and-Mouth Disease/*diagnosis;
Foot-and-Mouth Disease Virus/genetics;
Nucleic Acid Amplification Techniques/*methods/veterinary;
Reverse Transcriptase Polymerase Chain Reaction/veterinary;
Reverse Transcription/genetics;
Sensitivity and Specificity
- From:Journal of Veterinary Science
2014;15(3):423-426
- CountryRepublic of Korea
- Language:English
-
Abstract:
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to rapidly detect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples, sensitivity of the FMDV C RT-LAMP was found to be 10 times higher than that of conventional reverse transcription-PCR (RT-PCR). No cross-reactivity with A, Asia 1, or O FMDV or swine vesicular disease virus (SVDV) indicated that FMDV C RT-LAMP may be an exciting novel method for detecting FMDV C.
