Quantitative measurement of influenza virus replication using consecutive bronchoalveolar lavage in the lower respiratory tract of a ferret model.
10.4142/jvs.2014.15.3.439
- Author:
Dong Hun LEE
1
;
Jong In KIM
;
Jae Won LEE
;
Wook Hun CHUNG
;
Jae Keun PARK
;
Yu Na LEE
;
Jin Soo HAN
;
Hwi Yool KIM
;
Sang Won LEE
;
Chang Seon SONG
Author Information
1. Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea. songcs@konkuk.ac.kr
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
bronchoalveolar lavage;
ferret;
infection;
influenza
- MeSH:
Animals;
Bronchoalveolar Lavage/*veterinary;
Disease Models, Animal;
Female;
Ferrets/*virology;
Influenza A Virus, H3N2 Subtype/*physiology;
Orthomyxoviridae Infections/*veterinary/virology;
Respiratory System/*virology;
Virus Replication/*physiology
- From:Journal of Veterinary Science
2014;15(3):439-442
- CountryRepublic of Korea
- Language:English
-
Abstract:
The ferret is an established animal model of influenza virus infection. Although viral replication in the upper respiratory tract is usually measured with consecutively collected nasal washes, daily evaluation of viral replication in the lung is limited because a large numbers of ferrets need to be sacrificed at consecutive time points. To overcome this limitation, we performed a virus quantification assay using bronchoalveolar lavage (BAL) fluid. This non-invasive BAL technique allows consecutive quantification of virus replication in the lungs of living ferrets. Our method can be used for the longitudinal evaluation of virus tropism in the lower respiratory tract.
