Action Mechanism of Chamaecyparis obtusa Oil on Hair Growth.
- Author:
Young Ok PARK
1
;
Su Eun KIM
;
Young Chul KIM
Author Information
1. Department of Public Health, Graduate School, Keimyung University, Daegu, Korea. yckim@kmu.ac.kr
- Publication Type:Original Article
- Keywords:
Action mechanism;
Anagen phase;
C57BL/6 mice;
Chamaecyparis obtusa oil;
Hair growth
- MeSH:
Alkaline Phosphatase;
Animals;
Chamaecyparis*;
Epidermal Growth Factor;
Epidermis;
gamma-Glutamyltransferase;
Hair Follicle;
Hair*;
Immunohistochemistry;
Insulin-Like Growth Factor I;
Mice;
Minoxidil;
RNA, Messenger;
Skin;
Stem Cell Factor;
Vascular Endothelial Growth Factor A
- From:Toxicological Research
2013;29(4):241-247
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was carried out to examine the action mechanism of Chamaecyparis obtusa oil (CO) on hair growth in C57BL/6 mice. For alkaline phosphatase (ALP) and gamma-glutamyl transpeptidase (gamma-GT) activities in the skin tissue, at week 4, the 3% minoxidil (MXD) and 3% CO treatment groups showed an ALP activity that was significantly higher by 85% (p < 0.001) and 48% (p < 0.05) and an gamma-GT activity that was significantly higher by 294% (p < 0.01) and 254% (p < 0.05) respectively, as compared to the saline (SA) treatment group. For insulin-like growth factor-1 (IGF-1) mRNA expression in the skin tissue, at week 4, the MXD and CO groups showed a significantly higher expression by 204% (p < 0.05) and 426% (p < 0.01) respectively, as compared to the SA group. At week 4, vascular endothelial growth factor (VEGF) expression in the MXD and CO groups showed a significantly higher expression by 74% and 96% (p < 0.05) respectively, however, epidermal growth factor (EGF) expression in the MXD and CO groups showed a significantly lower expression by 66% and 61% (p < 0.05) respectively, as compared to the SA group. Stem cell factor (SCF) expression in the MXD and CO groups was observed by immunohistochemistry as significant in a part of the bulge around the hair follicle and in a part of the basal layer of the epidermis. Taking all the results together, on the basis of effects on ALP and gamma-GT activity, and the expression of IGF-1, VEGF and SCF, which are related to the promotion of hair growth, it can be concluded that CO induced a proliferation and division of hair follicle cells and maintained the anagen phase. Because EGF expression was decreased significantly, CO could delay the transition to the catagen phase.
