Transforming Growth Factor-beta is a Possible Paracrine Mediator in the Human Endometrial Decidualization.
- Author:
Dong Wook PARK
1
;
Dong Soon CHOI
;
Mi Ran KIM
;
Kyung Joo HWANG
;
Mi Yeong JO
;
Seong Hee AHN
;
Churl K MIN
;
Hee Sug RYU
Author Information
1. Department Molecular Science and Technology, Ajou University, Suwon, Korea.
- Publication Type:Original Article
- Keywords:
Decidualization;
TGF-beta;
HGF;
Co-culture;
Paracrine
- MeSH:
Cell Communication;
Cell Culture Techniques;
Coculture Techniques;
Collagen;
Culture Media, Conditioned;
Enzyme-Linked Immunosorbent Assay;
Epithelial Cells;
Estrogens;
Hepatocyte Growth Factor;
Humans*;
Progesterone;
Prolactin;
RNA, Messenger;
Stromal Cells;
Transforming Growth Factor beta;
Transforming Growth Factor beta1
- From:Korean Journal of Fertility and Sterility
2003;30(1):65-75
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: To investigate the role of TGF (Transforming growth factor-beta) involved in the paracrinic communication during decidualization between UEC (uterine epithelial cells) and USC (uterine stromal cells), we have employed a co-culture system composed of human endometrial epithelial and stromal cells in defined hormonal conditions. DESiGN: in the co-culture, endometrial epithelial cells cultured in the matrigel-coated cell culture insert are seeded on top of the endometrial stromal cells cultured within a collagen gel. The co-culture was maintained for 48 hours under the following hormonal conditions: progesterone dominant condition (100 nM P4 and 1 nM E2) or estrogen-dominant condition (100 nM E2 and 1 nM P4). 10 ng/ ml HGF and/or 10 ng/ml TGF-beta1 are added. METHODS: RT-PCR is utilized to detect mRNAs quantitatively. Enzyme-linked immunosorbent assay (ELiSA) and immunohistochemical staining are utilized to detect proteins in the tissue. RESULTS: Prolactin mRNA is expressed in the co-cultured stromal cells under the progesterone dominant condition. TGF-beta1 and its receptors are expressed in both the co-cultured epithelial and stromal cells irrespective of the steroid present, which is in contrast with no or negligible expression of TGF-beta1 or its receptor in cells separately cultured. Both estrogen and progesterone significantly elevate the concentration of hepatocyte growth factor (HGF) in the conditioned medium of the co-culture with the value of 4,325 pg/ml in E2-dominant and 2,000 pg/ml in P4-dominant condition compare to 150 pg/ ml in no hormone. in separately cultured stromal cells, administration of HGF induces the expression of TGF receptor 1 in both hormonal conditions, but induction of TGF receptor 2 is only manifest in the P4-dominant condition. Administration of TGF-beta and HGF directly induce the decidualization marker prolactin mRNA in separately cultured stromal cells. CONCLUSION: it is likely that steroid hormones induces prolactin mRNA indirectly by promoting the cell to cell communication between the stromal and the epithelial cells. TGF-beta and HGF are two possible paracrine mediators in the human endometrial decidualization.
