Expression of Apoptotic Genes in Mouse Preimplantation Embryo Development.
- Author:
Yu il LEE
1
;
Jin LEE
;
Mi Young KIM
;
Sang Young CHUN
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Chonnam National University, Gwangju, Korea. leeyi@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Blastocyst;
Culture medium;
Apoptotic gene;
Preimplantation embryo
- MeSH:
Animals;
Blastocyst*;
Culture Media;
Embryonic Development*;
Embryonic Structures;
Female;
Gene Expression;
Humans;
Mice*;
Morula;
Pregnancy;
RNA, Messenger
- From:Korean Journal of Fertility and Sterility
2003;30(1):77-84
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: The aim of this study was to evaluate the influence of three different media on preimplatation embryo development and the expression of Bcl-2, Mcl-1, Bax, and Bok in mouse. MATERiALS AND METHODS: Two-cell embryos were retrieved from iCR female mice (4 weeks old) at 48 hr after hCG injection and cultured in Ham's F-10, HTF, and G1.2 media. The developmental rate of 2-cell embryos was evaluated from 24 hr to 72 hr after culture. RT-PCR was performed for the detection of Bcl-2, Mcl-1, Bax, and Bok gene expression. RESULTS: The rates of morula and blastocyst in HTF and G1.2 media (88%, 98.1%) were significantly higher than those in Ham's F-10 media (39.6%) at 48 hr. Likewise, the rates of hatching and hatched blastocyst in HTF and G1.2 media (21.9%, 52.9%) were higher than those in Ham's F-10 media (3.5%) at 72 hr. Bcl-2 and Bax mRNAs were highly detected in embryos cultured in Ham's F-10 when compared in embryos cultured in HTF and G1.2. in contrast, the expression of Mcl-1 and Bok was not significantly different. CONCLUSION: These results show that HTF and G1.2 culture media increase the rate of blastocyst formation and stimulate Bcl-2 and Bax gene expression in mouse preimplantation embryos.
