Analysis of trimethylsilyl derivatization products of phosphatidylethanol by gas chromatography-mass spectrometry.
- Author:
Chang Suek YON
1
;
Joong Soo HAN
Author Information
1. Institute of Biomedical Science and Department of Biochemistry, College of Medicine, Hanyang University, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
GC-MS;
phosphatidylethanol;
trimethylsilyl derivative;
BSTFA
- MeSH:
Glycerophospholipids/*chemistry;
Mass Fragmentography/*methods;
Support, Non-U.S. Gov't;
Trimethylsilyl Compounds/*chemistry
- From:Experimental & Molecular Medicine
2000;32(4):243-245
- CountryRepublic of Korea
- Language:English
-
Abstract:
For the detection of rare phospholipid, phosphatidylethanol (PEt), GC-MS analysis method was adopted for the detection of derivatization products of PEt by N,O-bis (trimethylsilyl) trifluroacetamide (BSTFA). A re-structured molecule derived from PEt, ethyl bis (trimethylsilyl)-phosphate was found from search of Wiley database. This molecule can be used as a marker for PEt analysis. The molecular formula was C8H23O4PSi2 and weight of the formula was 270.09.
