Chlorogenic Acid Decreases Retinal Vascular Hyperpermeability in Diabetic Rat Model.
10.3346/jkms.2013.28.4.608
- Author:
Joo Young SHIN
1
;
Joonhong SOHN
;
Kyu Hyung PARK
Author Information
1. Department of Ophthalmology, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Chlorogenic Acid;
Diabetic Retinopathy;
Diabetes Rat Model;
Vascular Endothelial Growth Factor;
Blood-Retinal Barrier
- MeSH:
Animals;
Blood-Retinal Barrier/*drug effects;
Chlorogenic Acid/metabolism/*pharmacology;
Claudin-5/metabolism;
Dextrans/chemistry;
Diabetes Mellitus, Experimental/complications/metabolism/*pathology;
Diabetic Retinopathy/etiology/prevention & control;
Down-Regulation;
Fluorescein-5-isothiocyanate/chemistry;
Male;
Occludin/metabolism;
Rats;
Rats, Sprague-Dawley;
Retina/*metabolism;
Tight Junction Proteins/metabolism;
Vascular Endothelial Growth Factor A/metabolism;
Zonula Occludens-1 Protein/metabolism
- From:Journal of Korean Medical Science
2013;28(4):608-613
- CountryRepublic of Korea
- Language:English
-
Abstract:
To evaluate the effect of chlorogenic acid (CGA), a polyphenol abundant in coffee, on retinal vascular leakage in the rat model of diabetic retinopathy, Sprague-Dawley rats were divided into four groups: controls, streptozotocin-induced diabetic rats, and diabetic rats treated with 10 and 20 mg/kg chlorogenic acid intraperitoneally daily for 14 days, respectively. Blood-retinal barrier (BRB) breakdown was evaluated using FITC-dextran. Vascular endothelial growth factor (VEGF) distribution and expression level was evaluated with immunohistochemistry and Western blot analysis. Expression of tight junction proteins, occludin and claudin-5, and zonula occludens protein, ZO-1 was also evaluated with immunohistochemistry and Western blot analysis. BRB breakdown and increased vascular leakage was found in diabetic rats, with increased VEGF expression and down-regulation of occludin, claudin-5, and ZO-1. CGA treatment effectively preserved the expression of occludin, and decreased VEGF levels, leading to less BRB breakdown and less vascular leakage. CGA may have a preventive role in BRB breakdown in diabetic retinopathy by preserving tight junction protein levels and low VEGF levels.
