Preclinical Study of Cell Therapy for Osteonecrosis of the Femoral Head with Allogenic Peripheral Blood-Derived Mesenchymal Stem Cells.
10.3349/ymj.2016.57.4.1006
- Author:
Qiang FU
1
;
Ning Ning TANG
;
Qian ZHANG
;
Yi LIU
;
Jia Chen PENG
;
Ning FANG
;
Li Mei YU
;
Jin Wei LIU
;
Tao ZHANG
Author Information
1. Key Laboratory of Cell Engineering of Guizhou Province, The Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou, China. oceanzt@163.com
- Publication Type:Original Article
- Keywords:
Osteonecrosis of the femoral head (ONFH);
peripheral blood;
mesenchymal stem cells (MSCs);
cell transplantation;
disease model;
rabbit
- MeSH:
Animals;
Blood Cells/*cytology;
Bone Morphogenetic Protein 2/genetics;
*Cell- and Tissue-Based Therapy;
Femur Head Necrosis/metabolism/*pathology/*therapy;
Gene Expression Regulation;
*Mesenchymal Stem Cell Transplantation;
Mesenchymal Stromal Cells/*cytology;
Osteonecrosis/*pathology/*therapy;
PPAR gamma/genetics;
Rabbits;
Transplantation, Homologous
- From:Yonsei Medical Journal
2016;57(4):1006-1015
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To explore the value of transplanting peripheral blood-derived mesenchymal stem cells from allogenic rabbits (rPBMSCs) to treat osteonecrosis of the femoral head (ONFH). MATERIALS AND METHODS: rPBMSCs were separated/cultured from peripheral blood after granulocyte colony-stimulating factor mobilization. Afterwards, mobilized rPBMSCs from a second passage labeled with PKH26 were transplanted into rabbit ONFH models, which were established by liquid nitrogen freezing, to observe the effect of rPBMSCs on ONFH repair. Then, the mRNA expressions of BMP-2 and PPAR-γ in the femoral head were assessed by RT-PCR. RESULTS: After mobilization, the cultured rPBMSCs expressed mesenchymal markers of CD90, CD44, CD29, and CD105, but failed to express CD45, CD14, and CD34. The colony forming efficiency of mobilized rPBMSCs ranged from 2.8 to 10.8 per million peripheral mononuclear cells. After local transplantation, survival of the engrafted cells reached at least 8 weeks. Therein, BMP-2 was up-regulated, while PPAR-γ mRNA was down-regulated. Additionally, bone density and bone trabeculae tended to increase gradually. CONCLUSION: We confirmed that local transplantation of rPBMSCs benefits ONFH treatment and that the beneficial effects are related to the up-regulation of BMP-2 expression and the down-regulation of PPAR-γ expression.
