Apoptosis of the mitochondria protein p32 (gc1qbp) in human ovarian cancer cells.
- Author:
Miae WON
1
;
Sunyoung LEE
;
Sung Jo KIM
;
Seongmin YOON
;
Kangseok LEE
;
Jeong Jae KO
;
Jeehyeon BAE
Author Information
1. Graduate school of Life Science & Biotechnology, Pochon CHA University School of Medicine, Seongnam, Korea. jeehyeon@cha.ac.kr
- Publication Type:Original Article
- Keywords:
p32;
Harakiri;
Apoptosis;
Cell death;
Ovarian cancer
- MeSH:
Apoptosis;
Cell Death;
Cell Survival;
DNA;
Fluorescent Antibody Technique;
Humans;
Immunoprecipitation;
Mitochondria;
Ovarian Neoplasms;
Plasmids
- From:Korean Journal of Obstetrics and Gynecology
2008;51(8):858-865
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: The purpose of the study was to examine a possible physiological function of p32-mediated apoptosis signaling in ovarian cancer cells. METHODS: SK-OV-3 cells were transfected with respective plasmid DNAs, and cell viability was measured. By immunoprecipitation and immunofluorescence staining analysis, we confirmed that p32 interacts with Harakiri in ovarian cancer cells. RESULTS: In SK-OV-3 cells, p32 interacted with Harakiri and both p32 and Harakiri were colocalized in the mitochondria. In addition, overexpression of p32 induced apoptosis of ovarian cancer cells and augmented Harakiri-mediated apoptosis. CONCLUSION: Our results demonstrated p32 as an apoptosis inducer and helped to provide the better understanding of the function of p32 in ovarian cancer cells and a possibility of p32 in the application of cancer therapeutics.
