Expression of Local Immunosuppressive Factor, Indoleamine 2,3-dixygenase, in Human Coreal Cells.
10.3341/jkos.2007.48.8.1126-1133
- Author:
Yang Hwan RYU
1
;
Jae Chan KIM
Author Information
1. Department of Ophthalmology, College of Medicine, Chung-Ang University, Seoul, Korea. jck50ey@kornet.net
- Publication Type:Original Article
- Keywords:
Indoleamine 2,3-dioxygenase;
Corneal fibroblasts;
Immune privilege
- MeSH:
Apoptosis;
Blotting, Western;
Enzyme-Linked Immunosorbent Assay;
Epithelial Cells;
Fibroblasts;
Humans*;
Immune Tolerance;
Indoleamine-Pyrrole 2,3,-Dioxygenase;
RNA, Messenger;
Transplants
- From:Journal of the Korean Ophthalmological Society
2007;48(8):1126-1133
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To identify the localization of indoleamine 2,3-dioxygenase (IDO) in human corneal cells and to evaluate its ability to act as a local immunosuppressive factor. METHODS: The expression profile of IDO was obtained with RT-PCR and Western blot of in a primary culture of human corneal cells (fibroblasts, epithelial cells and endothelial cells). In order to investigate the immunosuppressive function of IDO, immune cells were cultured in a human corneal cell-conditioned medium, and their prolifleration was identified by the MTT assay. Moreover, apoptotic effects of IDO in immune cells treated with IFN-gamma were also investigated with apoptosis ELISA. RESULTS: Among the three different types of human corneal cells analyzed, mRNA and protein expression of IDO was observed only in human corneal fibroblasts. Immune cells cultured in a human corneal fibroblast-conditioned medium showed inhibited proliferation. Moreover, IFN-gamma-induced expression of IDO significantly enhanced apoptotic ability in a dose-depandant manner. CONCLUSIONS: Our results suggest that human corneal fibroblasts are relatively immuno-resistant and that expression of IDO may be one of the factors involved in the immune tolerance observed in corneal grafts.
