Identification of P2Y11 Receptor Expressed in Human Retinoblastoma Cells.
10.3341/jkos.2007.48.8.1134-1142
- Author:
Yoon Hee KIM
1
;
Dae Ran KIM
;
Ko I CHUN
;
Jong Hyuck LEE
;
In Deok KONG
Author Information
1. Shimmian Oculoplastic Center, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Calcium;
P2Y11 receptor;
Retinoblastoma cell
- MeSH:
Adenosine Triphosphate;
Calcium;
Cell Proliferation;
Humans*;
Pathologic Processes;
Retinoblastoma*;
RNA, Messenger
- From:Journal of the Korean Ophthalmological Society
2007;48(8):1134-1142
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The present study aimed to identify the characteristics and physiological function of the P2Y11 receptor, a receptor likely expressed in human retinoblastoma cells. METHODS: We measured possible P2Y11 signaling in WERI-Rb-1 cells using a Ca2+ imaging technique and RT-PCR. RESULTS: 1) 10 micro M ATP elicited a strong but transient increase in Ca2+ in the WERI-Rb-1 cells, and this Ca2+ rise was well maintained after external Ca2+-depletion. 2) ATP-induced Ca2+ response arose entirely through Ca2+ mobilization. 3) P2Y11 agonist (BzATP, 100 micro M) increased Ca2+ by 31.2+/-3.7 % of ATP effect. 4) mRNA for P2Y11 subtype was identified using RT-PCR. CONCLUSIONS: P2Y11 purinergic activation can increase the intracellular calcium level through calcium mobilization in undifferentiated retinoblastoma cells, which may play an important role in cell proliferation, differentiation, and even pathologic processes.
