Cytotoxicity of resin-based root canal sealer, adseal.
10.5395/JKACD.2004.29.6.498
- Author:
Hee Jung KIM
1
;
Seung Ho BAEK
;
Woo Cheol LEE
;
Han Soo PARK
;
Kwang Shik BAE
Author Information
1. Department of Conservative Dentistry, College of Dentistry, Seoul National University, Korea. baeks@plaza.snu.ac.kr
- Publication Type:Original Article
- Keywords:
Cytotoxicity;
Agar diffusion test;
Root canal sealer;
Decolorization index;
Adseal
- MeSH:
Agar;
Atmosphere;
Calcium Hydroxide;
Cell Culture Techniques;
Dental Pulp Cavity*;
Diffusion;
Fibroblasts;
Glass;
Mass Screening
- From:Journal of Korean Academy of Conservative Dentistry
2004;29(6):498-503
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The properties of ideal root canal sealers include the ability of sealing the total root canal system and no toxic effects to periradicular tissues. Cytotoxicity test using cell culture is a common screening method for evaluation of the biocompatibility of root canal sealers. The purpose of this study was to investigate the cytotoxic effect of newly developed resin-based sealer (Adseal 1, 2, and 3) comparing with those commercial resin-based sealers (AH26 and AH Plus), ZOE-based sealers (Tubliseal EWT, Pulp Canal Sealer EWT) and calcium hydroxide based sealer (Sealapex). An indirect contact test of cytotoxicity by agar diffusion was performed according to the international standard ISO 10993-5. L929 fibroblast cells were incubated at 37degrees C in humidified 5% CO2-containing air atmosphere. The freshly mixed test materials were inserted into glass rings of internal diameter 5 mm and height 5 mm placed on the agar. After the 24 hrs incubation period, the decolorization zones around the test materials were assessed using an inverted microscope with a calibrated screen. A Decolorization Index was determined for each specimen. Adseal 1, 2, and 3 did not exert any cytotoxic effects, whereas AH26, AH Plus, Tubliseal EWT, Pulp Canal Sealer EWT, and Sealapex produced mild cytotoxicity.
