Suppressive Effect on Renal Inflammation by Vitamin A Combined with Antibiotics in a Rat Model of Pyelonephritis.
- Author:
Tae Kyong YUN
1
;
Seung Ju LEE
;
Yong Hyun CHO
Author Information
1. Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea. cyh0831@catholic. ac.kr
- Publication Type:Original Article
- Keywords:
Vitamin A;
Pyelonephritis;
Inflammation
- MeSH:
Animals;
Anti-Bacterial Agents*;
Ciprofloxacin;
Escherichia coli;
Inflammation*;
Kidney;
Models, Animal*;
Pyelonephritis*;
Rats*;
Urethra;
Urinary Bladder;
Vitamin A*;
Vitamins*;
Weights and Measures
- From:Korean Journal of Urology
2003;44(5):490-494
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We evaluated the suppressive effects of vitamin A, in combination with antibiotics, on the development of inflammation in an animal model of ascending pyelonephritis. MATERIALS AND METHODS: An inoculum, of 1x10(9) Escherichia coli (O2:K1:H(-) ) P69 organisms per ml, was instilled into rat bladders, and the urethras of each rat occluded for 4 hours. The individual treatments with ciprofloxacin (15mg/kg, twice a day for 5 days) or vitamin A (500 microgram/kg, once a day for 5 days), or a combination of the was initiated 72 hours after the bacterial inoculation. The rats' renal weights were measured and examinations of the bacterial culture performed. The kidneys were harvested 4 weeks post-infection, and assessed for histopathologic inflammation. RESULTS: The renal weights in all groups were decreased, with the exception of the combined treatment group (p<0.05). In the urine and kidney microbiological cultures there were no E.coli growths in either the antibiotic or combined treatment groups. From microscopic examinations, the antibiotic treatment of pyelonephritic rats, in combination with vitamin A, was shown to result in significantly less renal inflammation compared to the other experimental groups. CONCLUSIONS: Vitamin A therapy in combination with antiobiotics can significantly reduce the inflammation associated with ascending pyelonephritis.
